{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5151, "factors": [{"C100_A": {"compound": "cortisol", "dose": "100"}}, {"C100_A": {"compound": "cortisol", "dose": "100"}}, {"C100_A": {"compound": "cortisol", "dose": "100"}}, {"C200_A": {"compound": "cortisol", "dose": "200"}}, {"C200_A": {"compound": "cortisol", "dose": "200"}}, {"C200_A": {"compound": "cortisol", "dose": "200"}}, {"Control_A": {"compound": "none", "dose": "  "}}, {"Control_A": {"compound": "none", "dose": "  "}}, {"Control_A": {"compound": "none", "dose": "  "}}, {"Pulse_A": {"compound": "cortisol", "dose": "100 and 200 oscillating"}}, {"Pulse_A": {"compound": "cortisol", "dose": "100 and 200 oscillating"}}, {"Pulse_A": {"compound": "cortisol", "dose": "100 and 200 oscillating"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Glucocorticoids act through the glucocorticoid receptor (GR), a ligand activated transcription factor, to modulate gene expression...", "pubmed_id": 21267416, "geo_gse_id": "E-MEXP-2447", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["adrenal gland", "cell", "gland"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-human-hela-c-2", "geo_id_plat": "E-MEXP-2447_A-AFFY-44", "name": "Transcription profiling by array of human HeLa cells treated with pulsatile cortisol", "created": "Sep.22, 2014", "summary": "Glucocorticoids act through the glucocorticoid receptor (GR), a ligand activated transcription factor, to modulate gene expression profiles in target cells and tissues.  The principal physiological glucocorticoid in humans, cortisol, is released in a pulsatile fashion from the adrenal gland, which results in oscillations in blood concentrations, with a period of 1 - 2 hours.  The biological consequence of fluctuating cortisol concentrations has not been explored.  To identify the role of cortisol concentration oscillations for target cell response a flow-through cell culture system was developed.  Transcription profiling was performed in the absence and presence of cortisol. Cells were exposed to constant cortisol levels of 100ng/ml or 200 ng/ml or oscillating cortisol concentrations.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2447", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2447/samples/"}