ArrayExpress Uploaderarrayexpress_sidhumanCD8+CD28+none27CD8+CD28+tBHP22CD8+CD28-none27CD8+CD28-tBHP22CD8+CD28+none71CD8+CD28-none71CD8+CD28+tBHP74CD8+CD28-tBHP74CD8+CD28+none81CD8+CD28-none81CD8+CD28+none33CD8+CD28-none33CD8+CD28+tBHP64CD8+CD28-tBHP64CD8+CD28+tBHP25514604We isolated highly purified CD8+CD28+ and CD8+CD28- T cell populations from healthy young and elderly persons for gene expression...E-MEXP-2345/profile/8773/arrayexpressuploader315cellDec.12, 2014FalseE-MEXP-2345_A-AFFY-44transcription-profiling-of-cd8-t-cell-subsets-fromTranscription profiling of CD8 T cell subsets from healthy young and elderly personsSep.22, 2014We isolated highly purified CD8+CD28+ and CD8+CD28- T cell populations from healthy young and elderly persons for gene expression profiling using Affymetrix oligonucleotide microarrays. We demonstrate that the gene expression profile of CD8+CD28- T cells is very similar in young and elderly persons. In contrast, CD8+CD28+ in elderly differ from CD8+CD28+ in young persons. Hierarchical clustering revealed that CD8+CD28+ in elderly are located between CD8+CD28+ in young and CD8?CD28- (young and old) T cells regarding their differentiation state. Our study demonstrates a dichotomy of gene expression levels between CD8+CD28+ T cells in young and elderly persons but a similarity between CD8+CD28- T cells in young and elderly persons. As CD8+CD28+ T cells from elderly and young persons are distinct due to a different composition of the population, these results suggest that the gene expression profile does not depend on chronological age but depends on the differentiation state of the individual cell types.http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2345http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2345/samples/