{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"Tg1 D1 IL IFN": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "interferon-gamma", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D1 IL IFN": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "interferon-gamma", "GENOTYPE": "wild_type"}}, {"Tg1 D1 IL IFN": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "interferon-gamma", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D1 IL IFN": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "interferon-gamma", "GENOTYPE": "wild_type"}}, {"Tg1 D1 IL IFN": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "interferon-gamma", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D1 IL IFN": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "interferon-gamma", "GENOTYPE": "wild_type"}}, {"WT1 D1 IL TNF": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "wild_type"}}, {"Tg1 D1 IL TNF": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "DcR3 transgenic"}}, {"Tg1 D2 IL TNF": {"TIME": 48, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D1 IL TNF": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "wild_type"}}, {"Tg1 D2 IL TNF": {"TIME": 48, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "DcR3 transgenic"}}, {"Tg1 D1 IL TNF": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D2 IL TNF": {"TIME": 48, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "wild_type"}}, {"Tg1 D2 IL TNF": {"TIME": 48, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D1 IL TNF": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "wild_type"}}, {"Tg1 D1 IL TNF": {"TIME": 24, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "DcR3 transgenic"}}, {"WT1 D2 IL TNF": {"TIME": 48, "COMPOUND1": "IL-1beta", "COMPOUND2": "tumor necrosis factor alpha", "GENOTYPE": "wild_type"}}], "id": 8104, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "[u'The islet primary non-function (PNF) is a serious problem in islet transplantation. In this study, we investigated whether...", "geo_gse_id": "E-MEXP-2306", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 17, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-pancreatic-islets", "geo_id_plat": "E-MEXP-2306_A-AFFY-45", "name": "Transcription profiling of mouse pancreatic islets from wild type and DcR3 transgenic mice to investigate primary non-function (PNF) in islet transplantation", "created": "Nov.21, 2014", "summary": "[u'The islet primary non-function (PNF) is a serious problem in islet transplantation. In this study, we investigated whether DcR3-secreting transgenic (Tg) islets could reduce PNF. We generated transgenic mice expressing human DcR3. The transgenically expressed DcR3 protected islets from IFN-gama plus IL-1beta, or TNF-alpha plus IL-1beta-induced dysfunction and apoptosis in vitro. The Tg islets presented significantly reduced PNF after transplantation. ', {u'br': None}, {u'br': None}, u' Three independent batches of islet isolation were carried out. For each batch, islets were obtained from 4 Tg and 4 WT mice, and pooled respectively. The pooled islets (Tg or WT) were then divided into 4 groups: 2 were cultured in the presence of IFN-gamma (0.5 ug/ml) plus IL-1beta (0.5 ng/ml) with 1 harvested at 24 h and 1 harvested at 48 h; 2 were cultured in the presence of TNF-alpha (100 ng/ml) plus IL-1beta (0.5 ng/ml) with 1 harvested at 24 h and 1 harvested at 48 h.  Each treatment employed 3 chips using RNA from 3 different batches of islet isolation. For each treatment, genes with a mean signal strength difference above 2-fold between Tg and WT islets were selected for reverse PCR confirmation.']", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2306", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2306/samples/"}