ArrayExpress Uploader0mouse24IL-1betainterferon-gammaDcR3 transgenic24IL-1betainterferon-gammawild_type24IL-1betainterferon-gammaDcR3 transgenic24IL-1betainterferon-gammawild_type24IL-1betainterferon-gammaDcR3 transgenic24IL-1betainterferon-gammawild_type24IL-1betatumor necrosis factor alphawild_type24IL-1betatumor necrosis factor alphaDcR3 transgenic48IL-1betatumor necrosis factor alphaDcR3 transgenic24IL-1betatumor necrosis factor alphawild_type48IL-1betatumor necrosis factor alphaDcR3 transgenic24IL-1betatumor necrosis factor alphaDcR3 transgenic48IL-1betatumor necrosis factor alphawild_type48IL-1betatumor necrosis factor alphaDcR3 transgenic24IL-1betatumor necrosis factor alphawild_type24IL-1betatumor necrosis factor alphaDcR3 transgenic48IL-1betatumor necrosis factor alphawild_type8104arrayexpress_sid6[u'The islet primary non-function (PNF) is a serious problem in islet transplantation. In this study, we investigated whether...E-MEXP-2306/profile/8773/arrayexpressuploader417Dec.12, 2014Falsetranscription-profiling-of-mouse-pancreatic-isletsE-MEXP-2306_A-AFFY-45Transcription profiling of mouse pancreatic islets from wild type and DcR3 transgenic mice to investigate primary non-function (PNF) in islet transplantationNov.21, 2014[u'The islet primary non-function (PNF) is a serious problem in islet transplantation. In this study, we investigated whether DcR3-secreting transgenic (Tg) islets could reduce PNF. We generated transgenic mice expressing human DcR3. The transgenically expressed DcR3 protected islets from IFN-gama plus IL-1beta, or TNF-alpha plus IL-1beta-induced dysfunction and apoptosis in vitro. The Tg islets presented significantly reduced PNF after transplantation. ', {u'br': None}, {u'br': None}, u' Three independent batches of islet isolation were carried out. For each batch, islets were obtained from 4 Tg and 4 WT mice, and pooled respectively. The pooled islets (Tg or WT) were then divided into 4 groups: 2 were cultured in the presence of IFN-gamma (0.5 ug/ml) plus IL-1beta (0.5 ng/ml) with 1 harvested at 24 h and 1 harvested at 48 h; 2 were cultured in the presence of TNF-alpha (100 ng/ml) plus IL-1beta (0.5 ng/ml) with 1 harvested at 24 h and 1 harvested at 48 h. Each treatment employed 3 chips using RNA from 3 different batches of islet isolation. For each treatment, genes with a mean signal strength difference above 2-fold between Tg and WT islets were selected for reverse PCR confirmation.']http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2306http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2306/samples/