ArrayExpress Uploader05130prostate carcinoma24PC3dimethyl sulfoxide 0.1 volume percentepithelial cellprostate carcinoma24PC3iberin 15 micromolarepithelial cellprostate carcinoma24PC3sulforaphane 15 micromolarepithelial cellprostate carcinoma6PC35-azacytidine 20 micromolarepithelial cellprostate carcinoma6PC35-azacytidine 20 micromolar and TSA 0.33 micromolarepithelial cellprostate carcinoma6PC3dimethyl sulfoxide 0.1 volume percentepithelial cellprostate carcinoma6PC3genistein 20 micromolarepithelial cellprostate carcinoma6PC3iberin 3 micromolarepithelial cellprostate carcinoma6PC3sulforaphane 3 micromolarepithelial cellprostate carcinoma6PC3epigallocatechin gallate 20 micromolarepithelial cellbenign prostatic hyperplasia24primary cellsdimethyl sulfoxide 0.1 volume percentepithelial cellbenign prostatic hyperplasia24primary cellsdimethyl sulfoxide 0.1 volume percentepithelial cellbenign prostatic hyperplasia24primary cellsdimethyl sulfoxide 0.1 volume percentepithelial cellbenign prostatic hyperplasia24primary cellsiberin 15 micromolarepithelial cellbenign prostatic hyperplasia24primary cellsiberin 15 micromolarepithelial cellbenign prostatic hyperplasia24primary cellsiberin 15 micromolarepithelial cellbenign prostatic hyperplasia24primary cellssulforaphane 15 micromolarepithelial cellbenign prostatic hyperplasia24primary cellssulforaphane 15 micromolarepithelial cellbenign prostatic hyperplasia24primary cellssulforaphane 15 micromolarepithelial cellbenign prostatic hyperplasia6primary cells5-azacytidine 20 micromolarepithelial cellbenign prostatic hyperplasia6primary cellsdimethyl sulfoxide 0.1 volume percentepithelial cellbenign prostatic hyperplasia6primary cellsepigallocatechin gallate 20 micromolarepithelial cellbenign prostatic hyperplasia6primary cellsgenistein 20 micromolarepithelial cellbenign prostatic hyperplasia6primary cellsiberin 3 micromolarepithelial cellbenign prostatic hyperplasia6primary cellssulforaphane 3 micromolarepithelial cellbenign prostatic hyperplasia24primary cellsdimethyl sulfoxide 0.1 volume percentstromal cellbenign prostatic hyperplasia24primary cellsdimethyl sulfoxide 0.1 volume percentstromal cellbenign prostatic hyperplasia24primary cellsdimethyl sulfoxide 0.1 volume percentstromal cellbenign prostatic hyperplasia24primary cellsiberin 15 micromolarstromal cellbenign prostatic hyperplasia24primary cellsiberin 15 micromolarstromal cellbenign prostatic hyperplasia24primary cellsiberin 15 micromolarstromal cellbenign prostatic hyperplasia24primary cellssulforaphane 15 micromolarstromal cellbenign prostatic hyperplasia24primary cellssulforaphane 15 micromolarstromal cellbenign prostatic hyperplasia24primary cellssulforaphane 15 micromolarstromal cellbenign prostatic hyperplasia6primary cells5-azacytidine 20 micromolarstromal cellbenign prostatic hyperplasia6primary cellsdimethyl sulfoxide 0.1 volume percentstromal cellbenign prostatic hyperplasia6primary cellsepigallocatechin gallate 20 micromolarstromal cellbenign prostatic hyperplasia6primary cellsgenistein 20 micromolarstromal cellbenign prostatic hyperplasia6primary cellsiberin 3 micromolarstromal cellbenign prostatic hyperplasia6primary cellssulforaphane 3 micromolarstromal cellarrayexpress_sid4[u'BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from...19489030E-MEXP-2034/profile/8773/arrayexpressuploader540cancergenomeprostateprostate cancerDec.12, 2014Falsetranscription-profiling-by-array-of-human-primar-8E-MEXP-2034_A-AFFY-44Transcription profiling by array of human primary prostate epithelial and stromal cells after treatment with 4-methylsulphinylbutyl and 3-methylsulphinypropyl isothiocyanatesSep.22, 2014[u'BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from glucosinolates that accumulate in broccoli are potential dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) accumulates in heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) accumulates in sprouting broccoli. While, there are many studies regarding the biological activity of SF, there are few studies associated with IB. Moreover, the majority of studies have been undertaken with transformed cells, usually epithelial in origin, that have cancerous phenotypes. ', {u'br': None}, u'METHOD: Primary epithelial and stromal cells were derived from benign prostatic hyperplasia tissue. Affymetrix U133 Plus 2.0 whole genome arrays were used to quantify global gene expression changes in these cells, following exposure to physiologically appropriate concentrations of SF and IB. Ontology and pathway analyses were used to interpret results. Changes in expression of a subset of genes were confirmed by RT PCR.', {u'br': None}, u'RESULTS: Global gene expression profiling identified epithelial and stromal specific gene expression profiles. IB and SF induced different changes in gene expression in both epithelial and stromal cells but these changes were associated with similar functions', {u'br': None}, u'CONCLUSION: This data suggests that IB and SF both induce genes associated with cancer prevention, and IB should be further investigated as a potential chemo-preventative target. ', {u'br': None}]http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2034humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2034/samples/