Dataset: Transcription profiling by array of human primary prostate epithelial and stromal cells after treatment with 4-methylsulphinylbutyl and 3-methylsulphinypropyl isothiocyanates

[u'BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from...

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[u'BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from glucosinolates that accumulate in broccoli are potential dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) accumulates in heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) accumulates in sprouting broccoli. While, there are many studies regarding the biological activity of SF, there are few studies associated with IB. Moreover, the majority of studies have been undertaken with transformed cells, usually epithelial in origin, that have cancerous phenotypes. ', {u'br': None}, u'METHOD: Primary epithelial and stromal cells were derived from benign prostatic hyperplasia tissue. Affymetrix U133 Plus 2.0 whole genome arrays were used to quantify global gene expression changes in these cells, following exposure to physiologically appropriate concentrations of SF and IB. Ontology and pathway analyses were used to interpret results. Changes in expression of a subset of genes were confirmed by RT PCR.', {u'br': None}, u'RESULTS: Global gene expression profiling identified epithelial and stromal specific gene expression profiles. IB and SF induced different changes in gene expression in both epithelial and stromal cells but these changes were associated with similar functions', {u'br': None}, u'CONCLUSION: This data suggests that IB and SF both induce genes associated with cancer prevention, and IB should be further investigated as a potential chemo-preventative target. ', {u'br': None}]

Species:: human
Samples:: 40
Source:: E-MEXP-2034
PubMed:: 19489030
Updated:: Dec.12, 2014
Registered:: Sep.22, 2014

Factors: (via ArrayExpress)

Sample	disease state	cell line	cell type	treatment	time
PC3 24h control	prostate carcinoma	PC3	epithelial cell	dimethyl sulfoxide 0.1 volume percent	24
PC3 24h iberin	prostate carcinoma	PC3	epithelial cell	iberin 15 micromolar	24
PC3 24h sulforaphane	prostate carcinoma	PC3	epithelial cell	sulforaphane 15 micromolar	24
PC3 6d 5-aza	prostate carcinoma	PC3	epithelial cell	5-azacytidine 20 micromolar	6
PC3 6d 5-aza and TSA	prostate carcinoma	PC3	epithelial cell	5-azacytidine 20 micromolar and TSA 0.33 micromolar	6
PC3 6d control	prostate carcinoma	PC3	epithelial cell	dimethyl sulfoxide 0.1 volume percent	6
PC3 6d genistein	prostate carcinoma	PC3	epithelial cell	genistein 20 micromolar	6
PC3 6d iberin	prostate carcinoma	PC3	epithelial cell	iberin 3 micromolar	6
PC3 6d sulforaphane	prostate carcinoma	PC3	epithelial cell	sulforaphane 3 micromolar	6
PC3 6d EGCG	prostate carcinoma	PC3	epithelial cell	epigallocatechin gallate 20 micromolar	6
epithelial 24h control 1	benign prostatic hyperplasia	primary cells	epithelial cell	dimethyl sulfoxide 0.1 volume percent	24
epithelial 24h control 1	benign prostatic hyperplasia	primary cells	epithelial cell	dimethyl sulfoxide 0.1 volume percent	24
epithelial 24h control 1	benign prostatic hyperplasia	primary cells	epithelial cell	dimethyl sulfoxide 0.1 volume percent	24
epithelial 24h iberin 1	benign prostatic hyperplasia	primary cells	epithelial cell	iberin 15 micromolar	24
epithelial 24h iberin 1	benign prostatic hyperplasia	primary cells	epithelial cell	iberin 15 micromolar	24
epithelial 24h iberin 1	benign prostatic hyperplasia	primary cells	epithelial cell	iberin 15 micromolar	24
epithelial 24h Sulforaphane 1	benign prostatic hyperplasia	primary cells	epithelial cell	sulforaphane 15 micromolar	24
epithelial 24h Sulforaphane 1	benign prostatic hyperplasia	primary cells	epithelial cell	sulforaphane 15 micromolar	24
epithelial 24h Sulforaphane 1	benign prostatic hyperplasia	primary cells	epithelial cell	sulforaphane 15 micromolar	24
epithelial 6d 5-aza	benign prostatic hyperplasia	primary cells	epithelial cell	5-azacytidine 20 micromolar	6
epithelial 6d control	benign prostatic hyperplasia	primary cells	epithelial cell	dimethyl sulfoxide 0.1 volume percent	6
epithelial 6d EGCG	benign prostatic hyperplasia	primary cells	epithelial cell	epigallocatechin gallate 20 micromolar	6
epithelial 6d genistein	benign prostatic hyperplasia	primary cells	epithelial cell	genistein 20 micromolar	6
epithelial 6d iberin	benign prostatic hyperplasia	primary cells	epithelial cell	iberin 3 micromolar	6
epithelial 6d sulforaphane	benign prostatic hyperplasia	primary cells	epithelial cell	sulforaphane 3 micromolar	6
stroma 24h control 1	benign prostatic hyperplasia	primary cells	stromal cell	dimethyl sulfoxide 0.1 volume percent	24
stroma 24h control 1	benign prostatic hyperplasia	primary cells	stromal cell	dimethyl sulfoxide 0.1 volume percent	24
stroma 24h control 1	benign prostatic hyperplasia	primary cells	stromal cell	dimethyl sulfoxide 0.1 volume percent	24
stroma 24h iberin 1	benign prostatic hyperplasia	primary cells	stromal cell	iberin 15 micromolar	24
stroma 24h iberin 1	benign prostatic hyperplasia	primary cells	stromal cell	iberin 15 micromolar	24
stroma 24h iberin 1	benign prostatic hyperplasia	primary cells	stromal cell	iberin 15 micromolar	24
stroma 24h Sulforaphane 1	benign prostatic hyperplasia	primary cells	stromal cell	sulforaphane 15 micromolar	24
stroma 24h Sulforaphane 1	benign prostatic hyperplasia	primary cells	stromal cell	sulforaphane 15 micromolar	24
stroma 24h Sulforaphane 1	benign prostatic hyperplasia	primary cells	stromal cell	sulforaphane 15 micromolar	24
stroma 6d 5-aza	benign prostatic hyperplasia	primary cells	stromal cell	5-azacytidine 20 micromolar	6
stroma 6d control	benign prostatic hyperplasia	primary cells	stromal cell	dimethyl sulfoxide 0.1 volume percent	6
stroma 6d EGCG	benign prostatic hyperplasia	primary cells	stromal cell	epigallocatechin gallate 20 micromolar	6
stroma 6d genistein	benign prostatic hyperplasia	primary cells	stromal cell	genistein 20 micromolar	6
stroma 6d iberin	benign prostatic hyperplasia	primary cells	stromal cell	iberin 3 micromolar	6
stroma 6d sulforaphane	benign prostatic hyperplasia	primary cells	stromal cell	sulforaphane 3 micromolar	6