Dataset: Transcription profiling by array of human primary prostate epithelial and stromal cells after treatment with 4-methylsulphinylbutyl and 3-methylsulphinypropyl isothiocyanates
[u'BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from...
[u'BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from glucosinolates that accumulate in broccoli are potential dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) accumulates in heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) accumulates in sprouting broccoli. While, there are many studies regarding the biological activity of SF, there are few studies associated with IB. Moreover, the majority of studies have been undertaken with transformed cells, usually epithelial in origin, that have cancerous phenotypes. ', {u'br': None}, u'METHOD: Primary epithelial and stromal cells were derived from benign prostatic hyperplasia tissue. Affymetrix U133 Plus 2.0 whole genome arrays were used to quantify global gene expression changes in these cells, following exposure to physiologically appropriate concentrations of SF and IB. Ontology and pathway analyses were used to interpret results. Changes in expression of a subset of genes were confirmed by RT PCR.', {u'br': None}, u'RESULTS: Global gene expression profiling identified epithelial and stromal specific gene expression profiles. IB and SF induced different changes in gene expression in both epithelial and stromal cells but these changes were associated with similar functions', {u'br': None}, u'CONCLUSION: This data suggests that IB and SF both induce genes associated with cancer prevention, and IB should be further investigated as a potential chemo-preventative target. ', {u'br': None}]
- Species:
- human
- Samples:
- 40
- Source:
- E-MEXP-2034
- PubMed:
- 19489030
- Updated:
- Dec.12, 2014
- Registered:
- Sep.22, 2014
| Sample | disease state | cell line | treatment | cell type | time |
|---|---|---|---|---|---|
| PC3 24h control | prostate carcinoma | PC3 | dimethyl sulfoxide 0.1 volume percent | epithelial cell | 24 |
| PC3 24h iberin | prostate carcinoma | PC3 | iberin 15 micromolar | epithelial cell | 24 |
| PC3 24h sulforaphane | prostate carcinoma | PC3 | sulforaphane 15 micromolar | epithelial cell | 24 |
| PC3 6d 5-aza | prostate carcinoma | PC3 | 5-azacytidine 20 micromolar | epithelial cell | 6 |
| PC3 6d 5-aza and TSA | prostate carcinoma | PC3 | 5-azacytidine 20 micromolar and TSA 0.33 micromolar | epithelial cell | 6 |
| PC3 6d control | prostate carcinoma | PC3 | dimethyl sulfoxide 0.1 volume percent | epithelial cell | 6 |
| PC3 6d genistein | prostate carcinoma | PC3 | genistein 20 micromolar | epithelial cell | 6 |
| PC3 6d iberin | prostate carcinoma | PC3 | iberin 3 micromolar | epithelial cell | 6 |
| PC3 6d sulforaphane | prostate carcinoma | PC3 | sulforaphane 3 micromolar | epithelial cell | 6 |
| PC3 6d EGCG | prostate carcinoma | PC3 | epigallocatechin gallate 20 micromolar | epithelial cell | 6 |
| epithelial 24h control 1 | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | epithelial cell | 24 |
| epithelial 24h control 1 | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | epithelial cell | 24 |
| epithelial 24h control 1 | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | epithelial cell | 24 |
| epithelial 24h iberin 1 | benign prostatic hyperplasia | primary cells | iberin 15 micromolar | epithelial cell | 24 |
| epithelial 24h iberin 1 | benign prostatic hyperplasia | primary cells | iberin 15 micromolar | epithelial cell | 24 |
| epithelial 24h iberin 1 | benign prostatic hyperplasia | primary cells | iberin 15 micromolar | epithelial cell | 24 |
| epithelial 24h Sulforaphane 1 | benign prostatic hyperplasia | primary cells | sulforaphane 15 micromolar | epithelial cell | 24 |
| epithelial 24h Sulforaphane 1 | benign prostatic hyperplasia | primary cells | sulforaphane 15 micromolar | epithelial cell | 24 |
| epithelial 24h Sulforaphane 1 | benign prostatic hyperplasia | primary cells | sulforaphane 15 micromolar | epithelial cell | 24 |
| epithelial 6d 5-aza | benign prostatic hyperplasia | primary cells | 5-azacytidine 20 micromolar | epithelial cell | 6 |
| epithelial 6d control | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | epithelial cell | 6 |
| epithelial 6d EGCG | benign prostatic hyperplasia | primary cells | epigallocatechin gallate 20 micromolar | epithelial cell | 6 |
| epithelial 6d genistein | benign prostatic hyperplasia | primary cells | genistein 20 micromolar | epithelial cell | 6 |
| epithelial 6d iberin | benign prostatic hyperplasia | primary cells | iberin 3 micromolar | epithelial cell | 6 |
| epithelial 6d sulforaphane | benign prostatic hyperplasia | primary cells | sulforaphane 3 micromolar | epithelial cell | 6 |
| stroma 24h control 1 | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | stromal cell | 24 |
| stroma 24h control 1 | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | stromal cell | 24 |
| stroma 24h control 1 | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | stromal cell | 24 |
| stroma 24h iberin 1 | benign prostatic hyperplasia | primary cells | iberin 15 micromolar | stromal cell | 24 |
| stroma 24h iberin 1 | benign prostatic hyperplasia | primary cells | iberin 15 micromolar | stromal cell | 24 |
| stroma 24h iberin 1 | benign prostatic hyperplasia | primary cells | iberin 15 micromolar | stromal cell | 24 |
| stroma 24h Sulforaphane 1 | benign prostatic hyperplasia | primary cells | sulforaphane 15 micromolar | stromal cell | 24 |
| stroma 24h Sulforaphane 1 | benign prostatic hyperplasia | primary cells | sulforaphane 15 micromolar | stromal cell | 24 |
| stroma 24h Sulforaphane 1 | benign prostatic hyperplasia | primary cells | sulforaphane 15 micromolar | stromal cell | 24 |
| stroma 6d 5-aza | benign prostatic hyperplasia | primary cells | 5-azacytidine 20 micromolar | stromal cell | 6 |
| stroma 6d control | benign prostatic hyperplasia | primary cells | dimethyl sulfoxide 0.1 volume percent | stromal cell | 6 |
| stroma 6d EGCG | benign prostatic hyperplasia | primary cells | epigallocatechin gallate 20 micromolar | stromal cell | 6 |
| stroma 6d genistein | benign prostatic hyperplasia | primary cells | genistein 20 micromolar | stromal cell | 6 |
| stroma 6d iberin | benign prostatic hyperplasia | primary cells | iberin 3 micromolar | stromal cell | 6 |
| stroma 6d sulforaphane | benign prostatic hyperplasia | primary cells | sulforaphane 3 micromolar | stromal cell | 6 |