{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8061, "factors": [{"4470a": {"genotype": "dcrfl/fl; R26R-yfp"}}, {"4470a": {"genotype": "dcrfl/fl; R26R-yfp"}}, {"4470a": {"genotype": "dcrfl/fl; R26R-yfp"}}, {"4470a": {"genotype": "dcrfl/fl; R26R-yfp"}}, {"4470a": {"genotype": "dcrfl/fl; R26R-yfp"}}, {"4470a": {"genotype": "dcrfl/fl; R26R-yfp"}}, {"4483a": {"genotype": "dcrfl/+; R26R-yfp"}}, {"4483a": {"genotype": "dcrfl/+; R26R-yfp"}}, {"4483a": {"genotype": "dcrfl/+; R26R-yfp"}}, {"4483a": {"genotype": "dcrfl/+; R26R-yfp"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Abelson virus (v-Abl)-transformed pre-B cell lines from BM of dcrfl/fl; R26R-yfp (4470; 4475) and dcrfl/+; R26R-yfp mice (4483) are...", "pubmed_id": 18329371, "geo_gse_id": "E-MEXP-1455", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 10, "tags": ["cell", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-abl-pre-b-cell-li", "geo_id_plat": "E-MEXP-1455_A-AFFY-45", "name": "Transcription profiling of mouse Abl pre-B cell lines with conditional deletion of dicer", "created": "Nov.21, 2014", "summary": "Abelson virus (v-Abl)-transformed pre-B cell lines from BM of dcrfl/fl; R26R-yfp (4470; 4475) and dcrfl/+; R26R-yfp mice (4483) are treated with a transducible Cre protein (Tat-Cre) to induce deletion of the dicer-1 gene in vitro. Cre activity is conveniently monitored by concomitant expression of YFP. To obtain Dicer KO cells, YFP+ cells from dcrfl/fl; R26R-yfp (4470_YFP; 4475_YFP) cultures treated with Tat-cre were isolated by fluorescence-activated cell sorting (FACS) and further propagated in cell culture. As controls, we used non-deleted, YFP- cells sorted from dcrfl/fl; R26R-yfp cultures (4470; 4475) treated with Tat-cre, and YFP+ deleted cells from dcrfl/+; R26R-yfp cultures (4483_YFP). To validate this system, various cell populations were sorted and analyzed for Dicer protein and miRNA expression. In the Dicer KO pro-B cell lines, Western analysis indicated that Dicer protein was efficiently ablated, and Northern blots demonstrated that the levels of mature miR-17 and miR-191 were drastically reduced.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1455", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1455/samples/"}