ArrayExpress Uploader08054macrophageUpf2fl/fl;LysMCremacrophageUpf2fl/fl;LysMCremacrophageUpf2fl/fl;LysMCremacrophagewild type genotypemacrophagewild type genotypemacrophagewild type genotypethymocyteUpf2fl/fl;LckCrethymocyteUpf2fl/fl;LckCrethymocyteUpf2fl/fl;LckCrethymocytewild type genotypethymocytewild type genotypethymocytewild type genotypearrayexpress_sid6Knockdown experiments in transformed human cells, suggested that nonsense-mediated mRNA decay (NMD) components had extensive effects on...18483223E-MEXP-1371/profile/8773/arrayexpressuploader212cellDec.12, 2014Falsetranscription-profiling-of-mouse-t-cell-thymocytesE-MEXP-1371_A-AFFY-45Transcription profiling of mouse T-cell thymocytes and bone marrow-derived macrophages dervied from Upf2fl/fl;LysMCre and Upf2fl/fl;LckCre mice to test the importance of nonsense-mediated mRNA decay on global transcriptionNov.21, 2014Knockdown experiments in transformed human cells, suggested that nonsense-mediated mRNA decay (NMD) components had extensive effects on the regulation of a significant fraction of the transcriptome In order to test the importance of NMD on global transcription we subjected BMDMs and T-cell thymocytes derived from Upf2fl/fl;LysMCre and Upf2fl/fl;LckCre mice, respectively, to microarray-based gene expression profiling. Both these cell types display strong, if not complete, reduction of NMD as assayed by transfection with reporter constructs or sequencing of Tcrb transcripts. Upf2¯/¯ BMDMs displayed significant changes in the expression of 182 genes (234 probe sets) of which 179 were upregulated.http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1371mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1371/samples/