{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8054, "factors": [{"BMM_ko-rep1": {"cell type": "macrophage", "genotype": "Upf2fl/fl;LysMCre"}}, {"BMM_ko-rep1": {"cell type": "macrophage", "genotype": "Upf2fl/fl;LysMCre"}}, {"BMM_ko-rep1": {"cell type": "macrophage", "genotype": "Upf2fl/fl;LysMCre"}}, {"BMM_wt-rep1": {"cell type": "macrophage", "genotype": "wild type genotype"}}, {"BMM_wt-rep1": {"cell type": "macrophage", "genotype": "wild type genotype"}}, {"BMM_wt-rep1": {"cell type": "macrophage", "genotype": "wild type genotype"}}, {"Thy_ko-rep1": {"cell type": "thymocyte", "genotype": "Upf2fl/fl;LckCre"}}, {"Thy_ko-rep1": {"cell type": "thymocyte", "genotype": "Upf2fl/fl;LckCre"}}, {"Thy_ko-rep1": {"cell type": "thymocyte", "genotype": "Upf2fl/fl;LckCre"}}, {"Thy_wt-rep1": {"cell type": "thymocyte", "genotype": "wild type genotype"}}, {"Thy_wt-rep1": {"cell type": "thymocyte", "genotype": "wild type genotype"}}, {"Thy_wt-rep1": {"cell type": "thymocyte", "genotype": "wild type genotype"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Knockdown experiments in transformed human cells, suggested that nonsense-mediated mRNA decay (NMD) components had extensive effects on...", "pubmed_id": 18483223, "geo_gse_id": "E-MEXP-1371", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-t-cell-thymocytes", "geo_id_plat": "E-MEXP-1371_A-AFFY-45", "name": "Transcription profiling of mouse T-cell thymocytes and bone marrow-derived macrophages dervied from Upf2fl/fl;LysMCre and Upf2fl/fl;LckCre mice to test the importance of nonsense-mediated mRNA decay on global transcription", "created": "Nov.21, 2014", "summary": "Knockdown experiments in transformed human cells, suggested that nonsense-mediated mRNA decay (NMD) components had extensive effects on the regulation of a significant fraction of the transcriptome   In order to test the importance of NMD on global transcription we subjected BMDMs and T-cell thymocytes derived from Upf2fl/fl;LysMCre and Upf2fl/fl;LckCre mice, respectively, to microarray-based gene expression profiling. Both these cell types display strong, if not complete, reduction of NMD as assayed by transfection with reporter constructs or sequencing of Tcrb transcripts. Upf2\u00af/\u00af BMDMs displayed significant changes in the expression of 182 genes (234 probe sets) of which 179 were upregulated.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1371", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1371/samples/"}