{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"TBP-105Qa": {"genotype": "TBP105Q"}}, {"TBP-105Qa": {"genotype": "TBP105Q"}}, {"TBP-105Qa": {"genotype": "TBP105Q"}}, {"TBP-13Qa": {"genotype": "TBP13Q"}}, {"TBP-13Qa": {"genotype": "TBP13Q"}}, {"TBP-13Qa": {"genotype": "TBP13Q"}}, {"WTa": {"genotype": "wild type"}}, {"WTa": {"genotype": "wild type"}}, {"WTa": {"genotype": "wild type"}}], "id": 8049, "pop_total": 0, "platform": 6, "summary_wrapped": "[u'Transcription profiles were obtained for 2-month old mice. Three lines (WT, TBP-13Q12, and TBP105Q) were used in these experiments. ',...", "pubmed_id": 17994014, "geo_gse_id": "E-MEXP-1314", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["disease", "huntington disease", "protein", "spinocerebellar ataxia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-cerebellum-cells-from-w", "geo_id_plat": "E-MEXP-1314_A-AFFY-45", "name": "Transcription profiling of cerebellum cells from wild type mice or mice overexpressing TATA-binding protein (TBP) or a mutant version of TBP", "created": "Nov.21, 2014", "summary": "[u'Transcription profiles were obtained for 2-month old mice. Three lines (WT, TBP-13Q12, and TBP105Q) were used in these experiments. ', {u'br': None}, {u'br': None}, u' Wild-type and 13Q mice are normal. The 13Q mice overexpress the normal protein on the RNA but not protein level and do not show a phenotype.  The 105Q mice express a mutant version of the protein TBP and faithfully model the disease SCA17 (spinocerebellar ataxia-17, huntington disease-like 4, HDL4).   ', {u'br': None}, {u'br': None}, u' The constructs containing mixed CAG/CAA trinucleotide repeats (and encoding polyglutamine tracts) of variable length were made using a previously described method (Michalik A et al., Biotechniques, 2001). Briefly,  synthetic CAG/CAA oligonucleotides were subcloned into a cDNA construct of the normal mouse (13 CAG/CAA) TBP gene.  Because of the mixed nature of the repeat, its length is stable in mitotic and meiotic transmission.']", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1314", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-1314/samples/"}