ArrayExpress Uploader0mouse8033arrayexpress_sid6Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells...E-GEOD-9977/profile/8773/arrayexpressuploader024Dec.12, 2014Falsetranscription-profiling-of-mouse-nih-3t3-cells-t-2E-GEOD-9977_A-AFFY-45Transcription profiling of mouse NIH-3T3 cells treated with mock, interferon alpha or gamma for 1or 3hNov.21, 2014Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells compared to mock; We used microarrays to analyze the effects of 1 and 3h of IFNalpha and gamma treatment in total cellular RNA Experiment Overall Design: NIH-3T3 cells ( 5th to 15th passage after thawing) were split from confluent plates 24h before start of the experiment. At the begin of the experiment about 80% confluency was reached. The experiment was started by applying fresh, prewarmed, CO2-equilibrated medium containing either mock, 100U/ml IFN alpha or 100 U/ml IFN gamma. Labeling was either started together with addition of IFN for 60 minutes or for 30 minutes starting 150 minutes after begin of treatment.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9977http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9977/samples/