{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE9977GSM252218": {}}, {"GSE9977GSM252219": {}}, {"GSE9977GSM252220": {}}, {"GSE9977GSM252221": {}}, {"GSE9977GSM252222": {}}, {"GSE9977GSM252223": {}}, {"GSE9977GSM252224": {}}, {"GSE9977GSM252225": {}}, {"GSE9977GSM252226": {}}, {"GSE9977GSM252227": {}}, {"GSE9977GSM252228": {}}, {"GSE9977GSM252229": {}}, {"GSE9977GSM252230": {}}, {"GSE9977GSM252231": {}}, {"GSE9977GSM252232": {}}, {"GSE9977GSM252233": {}}, {"GSE9977GSM252234": {}}, {"GSE9977GSM252235": {}}, {"GSE9977GSM252236": {}}, {"GSE9977GSM252237": {}}, {"GSE9977GSM252238": {}}, {"GSE9977GSM252239": {}}, {"GSE9977GSM252240": {}}, {"GSE9977GSM252241": {}}], "id": 8033, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells...", "geo_gse_id": "E-GEOD-9977", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 24, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-nih-3t3-cells-t-2", "geo_id_plat": "E-GEOD-9977_A-AFFY-45", "name": "Transcription profiling of mouse NIH-3T3 cells treated with mock, interferon alpha or gamma for 1or 3h", "created": "Nov.21, 2014", "summary": "Differential gene expression caused by 1h and 3h of IFN alpha or gamma treatment was analyzed in total cellular RNA of NIH-3T3 cells compared to mock; We used microarrays to analyze the effects of 1 and 3h of IFNalpha and gamma treatment in total cellular RNA Experiment Overall Design: NIH-3T3 cells ( 5th to 15th passage after thawing) were split from confluent plates 24h before start of the experiment. At the begin of the experiment about 80% confluency was reached. The experiment was started by applying fresh, prewarmed, CO2-equilibrated medium containing either mock, 100U/ml IFN alpha or 100 U/ml IFN gamma. Labeling was either started together with addition of IFN for 60 minutes or for 30 minutes starting 150 minutes after begin of treatment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9977", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9977/samples/"}