{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE9975GSM252180": {}}, {"GSE9975GSM252181": {}}, {"GSE9975GSM252182": {}}, {"GSE9975GSM252183": {}}, {"GSE9975GSM252184": {}}, {"GSE9975GSM252185": {}}, {"GSE9975GSM252186": {}}, {"GSE9975GSM252187": {}}, {"GSE9975GSM252188": {}}, {"GSE9975GSM252189": {}}, {"GSE9975GSM252190": {}}, {"GSE9975GSM252191": {}}, {"GSE9975GSM252192": {}}, {"GSE9975GSM252193": {}}, {"GSE9975GSM252194": {}}, {"GSE9975GSM252195": {}}, {"GSE9975GSM252196": {}}, {"GSE9975GSM252197": {}}, {"GSE9975GSM252198": {}}, {"GSE9975GSM252199": {}}, {"GSE9975GSM252200": {}}, {"GSE9975GSM252201": {}}, {"GSE9975GSM252202": {}}, {"GSE9975GSM252203": {}}, {"GSE9975GSM252204": {}}, {"GSE9975GSM252205": {}}, {"GSE9975GSM252206": {}}, {"GSE9975GSM252207": {}}, {"GSE9975GSM252208": {}}, {"GSE9975GSM252209": {}}, {"GSE9975GSM252210": {}}, {"GSE9975GSM252211": {}}, {"GSE9975GSM252212": {}}, {"GSE9975GSM252213": {}}, {"GSE9975GSM252214": {}}, {"GSE9975GSM252215": {}}], "id": 8032, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1 or 3h on nt-RNA labeled for 30-60 min at...", "geo_gse_id": "E-GEOD-9975", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 36, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-nih-3t3-cells-tre", "geo_id_plat": "E-GEOD-9975_A-AFFY-45", "name": "Transcription profiling of mouse NIH-3T3 cells treated with interferon alpha or gamma", "created": "Nov.21, 2014", "summary": "Expression data from NIH-3T3 cells treated with mock, 100 U/ml IFN alpha or 100 U/ml gamma for 1 or 3h on nt-RNA labeled for 30-60 min at different times of interferon treatment; Differential gene expression caused by IFN alpha or gamma was analyzed in newly transcribed RNA (nt-RNA) of NIH-3T3 cells treated for 1 or 3 h. RNA was labeled for 30 to 60 min and separated from total cellular RNA (tc-RNA) following Trizol RNA preparation and thiol-specific biotinylation; We used microarrays to analyze the effects of IFNalpha and gamma treatment in newly transcribed RNA (nt-RNA) Experiment Overall Design: NIH-3T3 cells (5th to 15th passage after thawing) were split 24 h before start of the experiment. When starting the experiment 80% confluency was reached. The experiment was started by applying fresh, prewarmed, CO2-equilibrated medium containing either mock, 100U/ml IFN alpha or 100 U/ml IFN gamma. Labeling was either started simultaneously, 30 or 150 minutes later.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9975", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9975/samples/"}