{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSE9974GSM252179": {}}, {"GSE9974GSM252175": {}}, {"GSE9974GSM252177": {}}, {"GSE9974GSM252178": {}}, {"GSE9974GSM252174": {}}, {"GSE9974GSM252176": {}}], "id": 5082, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Title: In vitro and in vivo effects of the orally bioavailable phenylbutyrate-derived histone deacetylase inhibitor OSU-HDAC42 on gene...", "geo_gse_id": "E-GEOD-9974", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["adenocarcinoma", "cancer", "genome", "histone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-9974_A-AFFY-44", "slug": "transcription-profiling-of-human-seg1-esophageal-c", "geo_gds_id": "", "name": "Transcription profiling of human SEG1 esophageal cell line treated with OSU-HDAC42, time series", "created": "Sep.22, 2014", "summary": "Title: In vitro and in vivo effects of the orally bioavailable phenylbutyrate-derived histone deacetylase inhibitor OSU-HDAC42 on gene expression in esophageal tissues and in esophageal adenocarcinoma cells; Histone deacetylases (HDACs) modulate nucleosomal packaging of DNA, thereby influencing gene transcription and multiple cancer-associated processes. Thus, we conducted microarray analysis at multiple time-points to assess the ability of OSU-HDAC42, the S enantiomer of the previously published compound HDAC-42,  to modulate key acid-induced changes as well as to impact other genes altered as the normal esophageal epithelium progresses along the metaplasia-dysplasia-esophageal adenocarcinoma continuum. Experiment Overall Design: SEG-1 cells were pretreated for 24 hours with vehicle or OSU-HDAC42, pulsed with acidified media (pH 3.5; 20 mins at 37C), replenished with media or OSU-HDAC42, and harvested 3, 6 and 24 hours later.  Global gene expression analysis was conducted using the human genome chip U133 2.0 Plus.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9974", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9974/samples/"}