{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 5076, "factors": [{"GSE9927GSM251195": {}}, {"GSE9927GSM251196": {}}, {"GSE9927GSM251197": {}}, {"GSE9927GSM251198": {}}, {"GSE9927GSM251199": {}}, {"GSE9927GSM251200": {}}, {"GSE9927GSM251207": {}}, {"GSE9927GSM251208": {}}, {"GSE9927GSM251209": {}}, {"GSE9927GSM251210": {}}, {"GSE9927GSM251211": {}}, {"GSE9927GSM251101": {}}, {"GSE9927GSM251105": {}}, {"GSE9927GSM251110": {}}, {"GSE9927GSM251111": {}}, {"GSE9927GSM251114": {}}, {"GSE9927GSM251126": {}}, {"GSE9927GSM251129": {}}, {"GSE9927GSM251192": {}}, {"GSE9927GSM251194": {}}], "pop_total": 0, "platform": 4, "summary_wrapped": "The mechanism of CD4(+) T cell depletion during chronic human immunodeficiency virus type 1 (HIV-1) infection remains unknown. Many...", "pubmed_id": 18077723, "geo_gse_id": "E-GEOD-9927", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 20, "tags": ["cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-9927_A-AFFY-44", "slug": "transcription-profiling-of-human-cd4-t-cells-from", "geo_gds_id": "", "name": "Transcription profiling of human CD4+ T cells from HIV infected individuals vs. controls reveals type I interferon-mediated disruption of T cell dynamics", "created": "Sep.22, 2014", "summary": "The mechanism of CD4(+) T cell depletion during chronic human immunodeficiency virus type 1 (HIV-1) infection remains unknown. Many studies suggest a significant role for chronic CD4(+) T cell activation. We assumed that the pathogenic process of excessive CD4(+) T cell activation would be reflected in the transcriptional profiles of activated CD4(+) T cells. Here we demonstrate that the transcriptional programs of in vivo activated CD4(+) T cells from untreated HIV(+) individuals are clearly different from those activated CD4(+) T cells from HIV(-) individuals. We observed a dramatic up-regulation of cell cycle-associated and interferon-stimulated transcripts in activated CD4(+) T cells of untreated HIV(+) individuals. Furthermore, we find an enrichment of proliferative and Type I interferon-responsive transcription factor binding sites in the promoters of genes that are differentially expressed in activated CD4(+) T cells of untreated HIV(+) individuals compared to HIV(-) individuals. We confirm these findings by examination of in vivo activated CD4(+) T cells. Taken together, these results suggest that activated CD4(+) T cells from untreated HIV(+) individuals are in a hyper-proliferative state that is modulated by Type I interferons. From these results, we propose a new model for CD4(+) T cell depletion during chronic HIV-1 infection. Experiment Overall Design: This experiment compares the expression of CD4+ T-cells obtained from 11 HIV+ individuals with that from comparable 9 HIV- control individuals.  Each individual's cells were analyzed on separate single-color chips, and the average values of both biological replicate groups were analyzed for statistical significance.   Experiment Overall Design: The biological significance of up- and down-regulated probesets/genes was analyzed using the Gene Ontology annotation dataset.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9927", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9927/samples/"}