{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8846, "factors": [{"GSE9727GSM245903": {}}, {"GSE9727GSM245904": {}}, {"GSE9727GSM245905": {}}, {"GSE9727GSM245897": {}}, {"GSE9727GSM245898": {}}, {"GSE9727GSM245899": {}}, {"GSE9727GSM245900": {}}, {"GSE9727GSM245901": {}}, {"GSE9727GSM245902": {}}, {"GSE9727GSM245893": {}}, {"GSE9727GSM245894": {}}, {"GSE9727GSM245895": {}}, {"GSE9727GSM245896": {}}, {"GSE9727GSM248228": {}}, {"GSE9727GSM248229": {}}, {"GSE9727GSM248230": {}}, {"GSE9727GSM248222": {}}, {"GSE9727GSM248223": {}}, {"GSE9727GSM248224": {}}, {"GSE9727GSM248225": {}}, {"GSE9727GSM248226": {}}, {"GSE9727GSM248227": {}}, {"GSE9727GSM248218": {}}, {"GSE9727GSM248219": {}}, {"GSE9727GSM248220": {}}, {"GSE9727GSM248221": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "The second messenger cAMP acts via protein kinase A (PKA) to induce apoptosis by mechanisms that are poorly understood. Here, we assessed...", "pubmed_id": 18048352, "geo_gse_id": "E-GEOD-9727", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 26, "tags": ["cell", "lymphoma", "membrane", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-s49-lymphoma-cell", "geo_id_plat": "E-GEOD-9727_A-AFFY-36", "name": "Transcription profiling of mouse S49 lymphoma cell deathless (D-) cell variant", "created": "Nov.24, 2014", "summary": "The second messenger cAMP acts via protein kinase A (PKA) to induce apoptosis by mechanisms that are poorly understood. Here, we assessed a role for mitochondria and analyzed gene expression in cAMP/PKA-promoted apoptosis by comparing wild-type (WT) S49 lymphoma cells and the S49 variant, D- (cAMP-deathless), which lacks cAMP-promoted apoptosis but has wild-type levels of PKA activity and cAMP-promoted G1 growth arrest. Treatment of WT, but not D-, S49 cells with 8-CPT-cAMP for 24 h induced loss of mitochondrial membrane potential, mitochondrial release of cytochrome c and Smac and increase in caspase-3 activity. Gene expression analysis (using Affymetrix 430 2.0  Arrays) revealed that WT and D- cells incubated with 8-CPT-cAMP have similar, but non-identical, extents of cAMP-regulated gene expression at 2h (~800 transcripts) and 6h (~1000 transcripts) (|Fold|>2, P<0.06); by contrast, at 24h ~2500 and ~1100 transcripts were changed in WT and D- cells, respectively. Using an approach that combined regression analysis, clustering and functional annotation to identify transcripts that showed differential expression between WT and D- cells, we found differences in cAMP-mediated regulation of mRNAs involved in transcriptional repression, apoptosis, the cell cycle, RNA splicing, Golgi and lysosomes. The 2 cell lines differed in CREB phosphorylation and expression of the transcriptional inhibitor Icer and in cAMP-regulated expression of genes in the Inhibitor of apoptosis (IAP) and Bcl families. The findings indicate that cAMP/PKA-promoted apoptosis of lymphoid cells occurs via mitochondrial-mediated events and imply that such apoptosis involves gene networks in multiple biochemical pathways. Experiment Overall Design: S49 D- cells were treated with 8-CPT-cAMP over the course of 24 hours. Cells were prepared for hybridization to microarrays at times 0-untreated, 2h, 6h, and 24h after treatment with 8-CPT-cAMP. Experimental replicates were as follows n=4 for time 0 and n=3 for 2, 6, and 24h post treatment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9727", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9727/samples/"}