ArrayExpress Uploader0mouseC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David GattasC57/BL6 (wild type)David Gattas8307arrayexpress_sid7To describe the transcriptional changes associated with polymicrobial-sepsis induced myocardial depression in wild type and iNOS...E-GEOD-9667/profile/8773/arrayexpressuploader29aortaarterycommon carotid arteryheartleftleft ventriclemyocardiumrightright common carotid arteryventricleDec.12, 2014Falsepolymicrobial-sepsis-induced-myocardial-depressionE-GEOD-9667_A-AFFY-23Polymicrobial sepsis-induced Myocardial Depression Transcriptional ProfileNov.23, 2014To describe the transcriptional changes associated with polymicrobial-sepsis induced myocardial depression in wild type and iNOS deficient mice. Keywords: myocardium, contractility, differential gene expression, nitric oxide synthase, infection We compared the transcriptional profile of C57/BL6 WT mice and congenic B6 129P2-Nos2tm1Lau/J mice after 48 hrs of polymicrobial sepsis induced by caecal ligation and perforation. 48 hours after surgery, mice were anaesthetised (intraperitoneal 100 mg/kg ketamine and 10 mg/kg xylazine). The right common carotid artery was cannulated (Millar Mikro-Tip pressure transducing catheter: 1.4F sensor, 2F catheter; Houston TX). Pressure tracings from the aorta and left ventricle were recorded (SonoLAB software; Sonometrics Corp., London Ontario Canada) and analysed using Cardiosoft and Origin 6.0 (Sonometrics Corp., and Microcal Software, Northampton MA). The heart was removed, emptied of blood, and snap frozen.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9667http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9667/samples/