Dataset: siRNA knockdown in human HEK293 cells transfected with siRNA specific for StarPap or PIPKIalpha or control siRNA

A loss of StarPap would be predicted to result in a decrease in cellular levels of mRNAs which it polyadenylates. Moreover, if PIPKIalpha has a function relationship with StarPap, knockdown of PIPKIalpha should cause a decrease in a pool of target mRNAs which require both StarPap and PIPKIalpha for their maturation. To test this, we independently knocked down StarPap and PIPKIalpha, and performed microarray analysis of total polyadenylated mRNAs from each group. Experiment Overall Design: HEK293 cells were transfected with siRNA specific for StarPap or PIPKIalpha or control siRNA. N=3

Species:

human

Samples:

9

Source:

E-GEOD-9361

PubMed:

18288197

Updated:

Dec.12, 2014

Registered:

Sep.22, 2014