{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7925, "factors": [{"GSE9037GSM229597": {}}, {"GSE9037GSM229598": {}}, {"GSE9037GSM229599": {}}, {"GSE9037GSM229600": {}}, {"GSE9037GSM229595": {}}, {"GSE9037GSM229596": {}}, {"GSE9037GSM229591": {}}, {"GSE9037GSM229592": {}}, {"GSE9037GSM229593": {}}, {"GSE9037GSM229594": {}}, {"GSE9037GSM229589": {}}, {"GSE9037GSM229590": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "IRAK-4 is an essential component of the signal transduction complex downstream of the IL-1- and Toll-like receptors. Though regarded as...", "pubmed_id": 18266302, "geo_gse_id": "E-GEOD-9037", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["bone", "bone marrow", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-bone-marrow-macro", "geo_id_plat": "E-GEOD-9037_A-AFFY-45", "name": "Transcription profiling of mouse bone marrow macrophages from WT and IRAK4 kinase", "created": "Nov.18, 2014", "summary": "IRAK-4 is an essential component of the signal transduction complex downstream of the IL-1- and Toll-like receptors. Though regarded as the first kinase in the signaling cascade, the role of IRAK-4 kinase activity versus its scaffold function is still controversial. In order to investigate the role of IRAK-4 kinase function in vivo, \u2018knock-in\u2019 mice were generated by replacing the wild type IRAK-4 gene with a mutant gene encoding kinase deficient IRAK-4 protein (IRAK-4 KD). Analysis of bone marrow macrophages obtained from WT and IRAK-4 KD mice with a number of experimental techniques demonstrated that the IRAK-4 KD cells greatly lack responsiveness to stimulation with the Toll-like receptor 4 (TLR4) agonist LPS. One of the techniques used, microarray analysis, identified IRAK-4 kinase-dependent LPS response genes and revealed that the induction of LPS-responsive mRNAs was largely ablated in IRAK-4 KD cells. In summary, our results suggest that IRAK-4 kinase activity plays a critical role in TLR4-mediated induction of inflammatory responses. Experiment Overall Design: The response of mouse bone marrow macrophages from WT and IRAK4 kinase dead animals to stimulation with LPS at two time points was determined. There were 12 samples in total, 6 from WT and 6 from IRAK4 kinase dead cells; for each strain there were 3 conditions: growth for 4 hours without stimulation (the strain-specific control), growth for 1 hour with stimulation, and growth for 4 hours with stimulation; for each condition there were two biological replicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9037", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-9037/samples/"}