{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE8828GSM219328": {}}, {"GSE8828GSM219329": {}}, {"GSE8828GSM219330": {}}, {"GSE8828GSM219325": {}}, {"GSE8828GSM219326": {}}, {"GSE8828GSM219327": {}}], "id": 7911, "pop_total": 0, "platform": 6, "summary_wrapped": "In human breast cancers, a phenotypically distinct minority population of tumorigenic cancer (TG) cells (sometimes referred to as cancer...", "pubmed_id": 17975224, "geo_gse_id": "E-GEOD-8828", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["breast", "breast cancer", "cancer", "cell", "compartment", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-8828_A-AFFY-45", "slug": "transcription-profiling-of-mouse-cancer-stem-cells", "geo_gds_id": "", "name": "Transcription profiling of mouse cancer stem cells in MMTVWnt-1 murine breast tumors", "created": "Nov.18, 2014", "summary": "In human breast cancers, a phenotypically distinct minority population of tumorigenic cancer (TG) cells (sometimes referred to as cancer stem cells) drives tumor growth when transplanted into immunodeficient mice. Our objective was to identify a mouse model of breast cancer stem cells that could have relevance to studying human breast cancer. To do so, we utilized breast tumors of the MMTVWnt-1 mice. MMTV-Wnt-1 breast tumors were harvested, dissociated into single cell suspensions, and FACS sorted on Thy1, CD24, and CD45. FACS sorted cells were then injected into recipient background FBV/NJ female mice. Thy1+CD24+ cancer cells, which constitute approximately 1-4% of tumor cells were highly enriched for cells capable of regenerating new tumors when compared to cells of the tumor that did not fit this profile (\u201cNot Thy1+CD24+\u201d). Resultant tumors were of the same phenotypic diversity as the original tumor and behaved in a similar manner when passaged. Microarray analysis comparing Thy1+CD24+ tumor cells to \u201cNot Thy1+CD24+\u201d cells identified a list of differentially expressed genes. Orthologs of these differentially expressed genes predicted survival of human breast cancer patients from two different study groups. These studies suggest that there is a cancer stem cell compartment in the MMTV-Wnt-1 murine breast tumor and that there is a clinical utility of this model for the study of cancer stem cells. Experiment Overall Design: Expression profling were performed on 3 tumorigenic and 3 non tumorigenic samples of MMTV-Wnt-1 breast tumors. A gene signature was derived by comparing the gene expressions of 3 tumorigenic samples with 3 nontumorigenic samples", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8828", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8828/samples/"}