{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSE8772GSM217874": {}}, {"GSE8772GSM217875": {}}, {"GSE8772GSM217876": {}}, {"GSE8772GSM217877": {}}, {"GSE8772GSM217878": {}}, {"GSE8772GSM217879": {}}], "id": 4994, "pop_total": 0, "platform": 4, "summary_wrapped": "Interference with chemoresistance to enhance the efficacy of chemotherapeutics may be of great utility for cancer therapy. We have...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["cancer", "cytokine", "melanoma", "point"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-8772_A-AFFY-44", "slug": "transcription-profiling-of-human-melanoma-cells-re", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8772", "geo_gds_id": "", "name": "Transcription profiling of human melanoma cells reveals KINK-1 a novel small-molecule inhibitor of IKK, enhances susceptibility of melanoma cells to antitumoral treatment", "created": "Sep.22, 2014", "summary": "Interference with chemoresistance to enhance the efficacy of chemotherapeutics may be of great utility for cancer therapy. We have identified KINK-1 (Kinase Inhibitor of NF-\uf06bB-1), a highly selective small-molecule IKK\uf062 inhibitor, as a potent suppressor of both constitutive and induced NF-\uf06bB activity in melanoma cells. While KINK-1 profoundly diminished various NF-\uf06bB-dependent gene products regulating proliferation, cytokine production or anti-apoptotic responses, the compound by itself showed little antiproliferative or pro-apoptotic activity on the cellular level. However, its combination with some cytostatics markedly enhanced their antitumoral activities in vitro, and doxorubicin-induced NF-\uf06bB activation, a mechanism implicated in chemoresistance, was abrogated by KINK-1. In addition, when KINK-1 was combined with doxorubicin in an in vivo melanoma model, experimental metastasis was significantly diminished as compared to either treatment alone. Induction of chemoresistance by KINK-1 in vivo was not observed. Thus, KINK-1 or related substances might increase the susceptibility of tumors to chemotherapy. Experiment Overall Design: one control and two time point (12hrs and 24hrs) are analyzed, with one replicate each (6 arrays total)", "geo_gse_id": "E-GEOD-8772", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8772/samples/"}