ArrayExpress Uploader0human4978arrayexpress_sid4In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and...E-GEOD-8608/profile/8773/arrayexpressuploader06chronic obstructive pulmonary diseasediseasemonocyteDec.12, 2014Falsetranscription-profiling-of-human-monocyte-derive-8E-GEOD-8608_A-AFFY-44Transcription profiling of human monocyte derived macrophages from COPD patients and healthy subjects after treatment with LPS or fine and ultrafine particlesSep.22, 2014In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and healthy subjects was investigated. MDM were treated with LPS, a combination of fine TiO2 and ultrafine Printex90 particles, or remained untreated. Experiment Overall Design: MDM of 13 COPD patients and 13 healthy subjects were incubated for four hours with LPS (10ng/ml), a combination of fine TiO2 and ultrafine Printex90 (32ug/ml each) or remained untreated. Cells were harvested, counted, and total RNA was isolated (phenol-chloroform extraction) for each subject individually. Total RNA of the 13 individuals from each group (COPD, healthy) and incubation (untreated, LPS, particles) were pooled and hybridized on a seperate array.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8608http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8608/samples/