{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSE8608GSM213465": {}}, {"GSE8608GSM213466": {}}, {"GSE8608GSM213464": {}}, {"GSE8608GSM213511": {}}, {"GSE8608GSM213512": {}}, {"GSE8608GSM213500": {}}], "id": 4978, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and...", "geo_gse_id": "E-GEOD-8608", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["chronic obstructive pulmonary disease", "disease", "monocyte"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-monocyte-derive-8", "geo_id_plat": "E-GEOD-8608_A-AFFY-44", "name": "Transcription profiling of human monocyte derived macrophages from COPD patients and healthy subjects after treatment with LPS or fine and ultrafine particles", "created": "Sep.22, 2014", "summary": "In this study gene expression of monocyte-derived macrophages (MDM) from chronic obstructive pulmonary disease (COPD) patients and healthy subjects was investigated. MDM were treated with LPS, a combination of fine TiO2 and ultrafine Printex90 particles, or remained untreated. Experiment Overall Design: MDM of 13 COPD patients and 13 healthy subjects were incubated for four hours with LPS (10ng/ml), a combination of fine TiO2 and ultrafine Printex90 (32ug/ml each) or remained untreated. Cells were harvested, counted, and total RNA was isolated (phenol-chloroform extraction) for each subject individually. Total RNA of the 13 individuals from each group (COPD, healthy) and incubation (untreated, LPS, particles) were pooled and hybridized on a seperate array.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8608", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8608/samples/"}