ArrayExpress Uploaderarrayexpress_sidmouse789206We compared gene expression profiles of aire-deficient and wild-type littermate thymic medullary epithelial cells. This was done in order...E-GEOD-8564/profile/8773/arrayexpressuploader020Dec.12, 2014FalseE-GEOD-8564_A-AFFY-45transcription-profiling-of-mouse-ire-deficient-andTranscription profiling of mouse ire-deficient and wild-type littermate thymic medullary epithelial cellsNov.18, 2014We compared gene expression profiles of aire-deficient and wild-type littermate thymic medullary epithelial cells. This was done in order to determine whether Aire's effects differed among strains, and also among individuals of the same strain. Experiment Overall Design: Individual thymi were digested with collagenase, dispase and DNAse, and meduallary epithelial cells with the phenotype CD45-G8.8+Ly51intMHCclasssIIhi were isolated by FACS.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8564http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8564/samples/