{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE8498GSM210755": {}}, {"GSE8498GSM210756": {}}, {"GSE8498GSM210757": {}}, {"GSE8498GSM210758": {}}, {"GSE8498GSM210759": {}}, {"GSE8498GSM210760": {}}], "id": 7888, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional...", "geo_gse_id": "E-GEOD-8498", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["exon", "nervous system", "neuron", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-cad-cells-transfe", "geo_id_plat": "E-GEOD-8498_A-AFFY-45", "name": "Transcription profiling of mouse CAD cells transfected with miR-124a-2 reveals the microRNA miR-124 Promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing", "created": "Nov.18, 2014", "summary": "Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124a directly targets PTBP1/PTB/hnRNPI mRNA, which encodes a global repressor of alternative pre-mRNA splicing in non-neuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2/nPTB/brPTB, an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay. During neuronal differentiation, miR-124a reduces PTBP1 levels leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124a plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124a promotes NS development at least in part by regulating an intricate network of NS-specific alternative splicing. We used microarrays to detail the global programme of gene expression of CAD cells over-expressing miR-124a-2. Experiment Overall Design: Expression data from CAD cells transfected with plasmid expressing miR-124a-2.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8498", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8498/samples/"}