{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE8396GSM207867": {}}, {"GSE8396GSM207868": {}}, {"GSE8396GSM207869": {}}, {"GSE8396GSM207870": {}}, {"GSE8396GSM207871": {}}, {"GSE8396GSM207820": {}}, {"GSE8396GSM207821": {}}, {"GSE8396GSM207822": {}}, {"GSE8396GSM207823": {}}, {"GSE8396GSM207824": {}}, {"GSE8396GSM207876": {}}, {"GSE8396GSM207877": {}}, {"GSE8396GSM207878": {}}, {"GSE8396GSM207879": {}}, {"GSE8396GSM207880": {}}, {"GSE8396GSM207886": {}}, {"GSE8396GSM207887": {}}, {"GSE8396GSM207888": {}}, {"GSE8396GSM207889": {}}, {"GSE8396GSM207890": {}}, {"GSE8396GSM207830": {}}, {"GSE8396GSM207831": {}}, {"GSE8396GSM207832": {}}, {"GSE8396GSM207833": {}}, {"GSE8396GSM207839": {}}, {"GSE8396GSM207840": {}}, {"GSE8396GSM207841": {}}, {"GSE8396GSM207842": {}}, {"GSE8396GSM207843": {}}, {"GSE8396GSM205778": {}}, {"GSE8396GSM205779": {}}, {"GSE8396GSM205780": {}}, {"GSE8396GSM205781": {}}, {"GSE8396GSM205782": {}}, {"GSE8396GSM207858": {}}, {"GSE8396GSM207859": {}}, {"GSE8396GSM207860": {}}, {"GSE8396GSM207861": {}}, {"GSE8396GSM205773": {}}, {"GSE8396GSM205774": {}}, {"GSE8396GSM205775": {}}, {"GSE8396GSM205776": {}}, {"GSE8396GSM205777": {}}, {"GSE8396GSM207849": {}}, {"GSE8396GSM207850": {}}, {"GSE8396GSM207851": {}}, {"GSE8396GSM207852": {}}, {"GSE8396GSM207853": {}}, {"GSE8396GSM207862": {}}, {"GSE8396GSM207863": {}}, {"GSE8396GSM207864": {}}, {"GSE8396GSM207865": {}}, {"GSE8396GSM207866": {}}, {"GSE8396GSM207815": {}}, {"GSE8396GSM207816": {}}, {"GSE8396GSM207817": {}}, {"GSE8396GSM207818": {}}, {"GSE8396GSM207819": {}}, {"GSE8396GSM207872": {}}, {"GSE8396GSM207873": {}}, {"GSE8396GSM207874": {}}, {"GSE8396GSM207875": {}}, {"GSE8396GSM207881": {}}, {"GSE8396GSM207882": {}}, {"GSE8396GSM207883": {}}, {"GSE8396GSM207884": {}}, {"GSE8396GSM207885": {}}, {"GSE8396GSM207825": {}}, {"GSE8396GSM207826": {}}, {"GSE8396GSM207827": {}}, {"GSE8396GSM207828": {}}, {"GSE8396GSM207829": {}}, {"GSE8396GSM207834": {}}, {"GSE8396GSM207835": {}}, {"GSE8396GSM207836": {}}, {"GSE8396GSM207837": {}}, {"GSE8396GSM207838": {}}, {"GSE8396GSM205769": {}}, {"GSE8396GSM205770": {}}, {"GSE8396GSM205771": {}}, {"GSE8396GSM205772": {}}, {"GSE8396GSM207854": {}}, {"GSE8396GSM207855": {}}, {"GSE8396GSM207856": {}}, {"GSE8396GSM207857": {}}, {"GSE8396GSM205766": {}}, {"GSE8396GSM205767": {}}, {"GSE8396GSM205768": {}}, {"GSE8396GSM207844": {}}, {"GSE8396GSM207845": {}}, {"GSE8396GSM207846": {}}, {"GSE8396GSM207847": {}}, {"GSE8396GSM207848": {}}], "id": 7883, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Dietary fatty acids have myriads of effects on human health and disease. Many of these effects are likely achieved by altering expression...", "geo_gse_id": "E-GEOD-8396", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 93, "tags": ["cellulose", "disease", "fatty acid", "genome", "liver"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-wild-type-129s1sv", "geo_id_plat": "E-GEOD-8396_A-AFFY-45", "name": "Transcription profiling of mouse wild-type (129S1/SvImJ) and PPARalpha -/- (129S4/SvJae) livers after feeding with synthetic dietary triglycerides:", "created": "Nov.18, 2014", "summary": "Dietary fatty acids have myriads of effects on human health and disease. Many of these effects are likely achieved by altering expression of genes. Several transcription factors have been shown to be responsive to fatty acids, including SREBP-1c, NF-kB, RXRs, LXRs, FXR, HNF4\u03b1, and PPARs. However, the relative importance of these transcription factors in regulation of gene expression by dietary fatty acids remains unclear. Here, we take advantage of a unique experimental design using synthetic triglycerides composed of one single fatty acid in combination with gene expression profiling to examine the acute effects of individual dietary fatty acids on hepatic gene expression in mice. The dietary interventions were performed in parallel in wild-type and PPAR\u03b1-/- mice, enabling the determination of the specific contribution of PPAR\u03b1. Depending on chain length and degree of saturation, dietary fatty acids caused a statistically significant change in expression of over 400 genes. Surprisingly, the far majority of genes regulated by dietary fatty acids in wild-type mice were unaltered in mice lacking PPAR\u03b1, indicating PPAR\u03b1-dependent regulation. We conclude that the effects of dietary fatty acids on hepatic gene expression are almost entirely mediated by PPAR\u03b1, indicating that PPAR\u03b1 dominates fatty acid-dependent gene regulation in liver. Experiment Overall Design: 2-6 months old male pure bred wild-type (129S1/SvImJ) and PPAR\u03b1 -/- (129S4/SvJae) mice were used. Experiment Overall Design: Wild-type and PPAR\u03b1 -/- mice fasted for 4 hours received a single dose of 400 \u00b5l synthetic triglyceride (tridecanoin \u2013 C10:0, triolein \u2013 C18:1, trilinolein \u2013 C18:2, trilinolenin \u2013 C18:3, trieicosapentaenoin \u2013 C20:5 or tridocosahexaenoin \u2013 C22:6), the synthetic PPAR\u03b1 agonists WY14643 or fenofibrate (400 \u03bcl of 10 mg/ml in 0.5% carboxymethyl cellulose, CMC) or control (400 \u03bcl of 0.5% CMC). 6 hours after gavage, mice were killed and livers extracted. Experiment Overall Design: (Please note: Experiments with C10:0, C18:2 and C18:3 were carried out a few months later than the rest of the treatments. Therefore there is a second set of control arrays \u2013 named control2 \u2013 which belong to these three treatment groups.) Experiment Overall Design: Liver total RNA from biological replicates was hybridized onto Affymetrix mouse genome 430 2.0 GeneChip arrays. Five microgram total RNA was labelled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix's protocols.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8396", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8396/samples/"}