{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE8291GSM205764": {}}, {"GSE8291GSM205763": {}}, {"GSE8291GSM205762": {}}, {"GSE8291GSM205761": {}}], "id": 8825, "pop_total": 0, "platform": 8, "summary_wrapped": "PPAR\u03b1 is a ligand-activated transcription factor involved in the regulation of nutrient metabolism and inflammation. Although much is...", "pubmed_id": 18288265, "geo_gse_id": "E-GEOD-8291", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["hormone", "lipid", "liver", "phosphatidylcholine", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-wild-type-and-p-2", "geo_id_plat": "E-GEOD-8291_A-AFFY-36", "name": "Transcription profiling of mouse wild-type and PPARI?-null liver treated with the synthetic PPARI? ligand WY14643", "created": "Nov.24, 2014", "summary": "PPAR\u03b1 is a ligand-activated transcription factor involved in the regulation of nutrient metabolism and inflammation. Although much is already known about the function of PPAR\u03b1 in hepatic lipid metabolism, many PPAR\u03b1-dependent pathways and genes have yet to be discovered.  In order to obtain an overview of PPAR\u03b1-regulated genes relevant to lipid metabolism, and to probe for novel candidate PPAR\u03b1 target genes, livers from several animal studies in which PPAR\u03b1 was activated and/or disabled were analyzed by Affymetrix GeneChips. Numerous novel PPAR\u03b1-regulated genes relevant to lipid metabolism were identified. Out of this set of genes, eight genes were singled out for study of PPAR\u03b1-dependent regulation in mouse liver and in mouse, rat, and human primary hepatocytes, including thioredoxin interacting protein (Txnip), electron-transferring-flavoprotein \u03b2 polypeptide (Etfb), electron-transferring-flavoprotein dehydrogenase (Etfdh), phosphatidylcholine transfer protein (Pctp), endothelial lipase (EL, Lipg), adipose triglyceride lipase (Pnpla2), hormone-sensitive lipase (Lipe), and monoglyceride lipase (Mgll). Using an in silico screening approach, one or more PPAR response elements (PPREs) were identified in each of these genes.  Since Pnpla2, Lipe, and Mgll contribute to hepatic triglyceride hydrolysis, gene regulation was studied under conditions of elevated hepatic lipids. In wild-type mice fed a high fat diet, the decrease in hepatic lipids following treatment with the PPAR\u03b1 agonist Wy14643 was paralleled by significant up-regulation of Pnpla2, Lipe, and Mgll, suggesting that induction of triglyceride hydrolysis may contribute to the anti-steatotic role of PPAR\u03b1. Our study illustrates the power of transcriptional profiling to uncover novel PPAR\u03b1-regulated genes and pathways in liver. Experiment Overall Design: 3-5 months old male pure bred wild-type (129S1/SvImJ) and PPAR\u03b1-null (129S4/SvJae) mice were used. Experiment Overall Design: Wild-type and PPAR\u03b1-null mice were treated with the synthetic PPAR\u03b1 ligand WY14643 (0.1% w/w) mixed in the food, or normal food (control) for 5 days (n=5 per group). Liver total RNA of pooled samples (within groups) was hybridized onto Affymetrix MOE430A GeneChip arrays. Experiment Overall Design: Five microgram total RNA was labelled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix's protocols.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8291", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8291/samples/"}