{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 1572, "factors": [{"GSE8096GSM200741": {}}, {"GSE8096GSM200757": {}}, {"GSE8096GSM200754": {}}, {"GSE8096GSM200740": {}}, {"GSE8096GSM200748": {}}, {"GSE8096GSM200755": {}}, {"GSE8096GSM200749": {}}, {"GSE8096GSM200743": {}}, {"GSE8096GSM200739": {}}, {"GSE8096GSM200612": {}}, {"GSE8096GSM200742": {}}, {"GSE8096GSM200756": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "Nonmalignant human mammary epithelial cells (HMEC) seeded in laminin-rich extracellular matrix (lrECM) form polarized acini and, in doing...", "pubmed_id": 16849555, "geo_gse_id": "E-GEOD-8096", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["breast", "breast cancer", "cancer", "laminin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-mammary-epithelia", "geo_id_plat": "E-GEOD-8096_A-AFFY-33", "name": "Transcription profiling of human mammary epithelial cell lines with finite life span and immortal non-malignant cells - time series", "created": "Jun.19, 2014", "summary": "Nonmalignant human mammary epithelial cells (HMEC) seeded in laminin-rich extracellular matrix (lrECM) form polarized acini and, in doing so, transit from a disorganized proliferating state to an organized growth-arrested state. We hypothesized that the gene expression pattern of organized and growth-arrested HMECs would share similarities with breast tumors with good prognoses. Using Affymetrix HG-U133A microarrays, we analyzed the expression of 22,283 gene transcripts in 184 (finite life span) and HMT3522 S1 (immortal nonmalignant) HMECs on successive days after seeding in a lrECM assay. Both HMECs underwent growth arrest in G0-G1 and differentiated into polarized acini between days 5 and 7. We identified gene expression changes with the same temporal pattern in both lines and examined the expression of these genes in a previously published panel of microarray data for 295 breast cancer samples. We show that genes that are significantly lower in the organized, growth-arrested HMEC than in their proliferating counterparts can be used to classify breast cancer patients into poor and good prognosis groups with high accuracy. This study represents a novel unsupervised approach to identifying breast cancer markers that may be of use clinically. Experiment Overall Design: We analyzed gene expression in 184 (finite life span) and HMT3522 S1 (immortal non-malignant) HMECs on successive days (3, 5, and 7) post-seeding in a laminin-rich extracellular matrix assay. Both HMECs underwent growth arrest in G0/G1 and differentiated into polarized acini between days 5 and 7.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8096", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8096/samples/"}