ArrayExpress Uploader04952GS-3GS-3GS-5GS-5GS-8GS-8GS-9GS-9GS-1GS-1GS-2GS-2GS-4GS-4GS-6GS-7GS-7arrayexpress_sid4Recent studies demonstrated that tumor cells with stem cell-like properties can be cultured from human glioblastomas by using conditions...18037961E-GEOD-8049/profile/8773/arrayexpressuploader117cellnervous systemstem cellDec.12, 2014Falsetranscription-profiling-by-array-of-human-gliobl-9E-GEOD-8049_A-AFFY-44Transcription profiling by array of human glioblastoma cell linesSep.22, 2014Recent studies demonstrated that tumor cells with stem cell-like properties can be cultured from human glioblastomas by using conditions that select for the expansion of neural stem cells. We established glioblastoma stem-like (GS-) cell cultures from 9 different glioblastomas, 8 of which generated stably expandable cell lines. Analyzing GS-cell cultures, we discovered two clearly discernable phenotypes. Microarray analysis showed that the 4 GSf cell lines shared expression profiles dominated by genes involved in nervous system development and neuropeptide signaling, while the 5 GSr lines shared expression signatures enriched for extracellular matrix-proteins. Experiment Overall Design: Affymetrix HG-U133 Plus 2.0 chips were employed for expression profiling from 9 different glioblasoma derived neurosphere cultures in two biological replicates.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8049humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-8049/samples/