ArrayExpress Uploader0mouse7849arrayexpress_sid6Comparisons of global gene-expression profiles revealed a greater distinction between CD4+ Treg cells and CD4+ conventional (Tconv) T...E-GEOD-7852/profile/8773/arrayexpressuploader018cellspleenthymusDec.12, 2014Falsetranscription-profiling-of-mouse-fat-derived-flowE-GEOD-7852_A-AFFY-45Transcription profiling of mouse fat derived flow sorted Treg cellsNov.18, 2014Comparisons of global gene-expression profiles revealed a greater distinction between CD4+ Treg cells and CD4+ conventional (Tconv) T cells residing in abdominal (epidydimal) fat versus in more standard locations such as the spleen, thymus and LN. Experiment Overall Design: All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 3-5 x 104 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7852http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7852/samples/