ArrayExpress Uploader0mouse7824arrayexpress_sid6The CD4+Foxp3+ regulatory T cells play an essential role in maintaining tolerance via their suppressive function on conventional T cells....E-GEOD-7596/profile/8773/arrayexpressuploader06axiscellDec.12, 2014Falsetranscription-profiling-of-mouse-t-cells-transfectE-GEOD-7596_A-AFFY-45Transcription profiling of mouse T cells transfected with constituatively actived Akt vs controls reveals AKT regulates de novo induction of Foxp3Nov.18, 2014The CD4+Foxp3+ regulatory T cells play an essential role in maintaining tolerance via their suppressive function on conventional T cells. The intracellular signaling pathways that regulate Foxp3 expression are largely unknown. In this study we describe a novel inhibitory role for AKT in regulating de novo induction of Foxp3 both in vivo and in vitro. A constitutively active allele of AKT significantly diminished TGF-â induced Foxp3 induction via a rapamycin-sensitive pathway, establishing a role for the AKT-mTOR axis in Treg cells. Moreover, the observed impairment in Foxp3 induction was paralleled by a selective downmodulation of the imparted Treg transcriptional signature highlighting the importance of the balance of intracellular signals in Treg differentiation . Our results provide a basis for further elucidation of molecular mechanisms that regulate Foxp3 induction and identify AKT as an important negative regulator of this process. Experiment Overall Design: All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 0.5-2.5 x 105 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7596http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7596/samples/