{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE7396GSM177575": {}}, {"GSE7396GSM177576": {}}, {"GSE7396GSM177573": {}}, {"GSE7396GSM177574": {}}], "id": 7816, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "We investigated the ability of transferrin receptor1 (TfRc) knockout cells to populate different domains of the developing kidney by...", "geo_gse_id": "E-GEOD-7396", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 4, "tags": ["blastocyst", "cell", "compartment", "genome", "kidney", "nephron", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-7396_A-AFFY-45", "slug": "transcription-profiling-of-mouse-tfrc-deficient-an", "geo_gds_id": "", "name": "Transcription profiling of mouse TfRc deficient and wild type renalcells in chimeric embryonic kidneys derived from embryos created by blastocyst injection", "created": "Nov.18, 2014", "summary": "We investigated the ability of transferrin receptor1 (TfRc) knockout cells to populate different domains of the developing kidney by using a chimeric approach. The TfRc cells developed into all segments of the developing nephron, but there was a relative exclusion from the ureteric bud and a positive bias towards the stromal compartment. Here we conducted a microarray analysis of differential gene expression between TfRc deficient and wild type (wt) cells in chimeric embryonic kidneys derived from embryos created by blastocyst injection of wt blastocysts with TfRc-/- green fluorescent protein-expressing (GFP+) embryonic stem cells. Experiment Overall Design: Following blastocyst injection of 3-5 TfRc-/- GFP+ embryonic stem cells in wt blstocysts, we harvested the chimeric kidney at day E15.5 and separated the TfRc-/- GFP+ cell population from wt GFP- cell population by using fluorescence activated cell sorting. Biotinlyated cRNA was prepared from the isolated cells and hybridized to Mouse Genome 430 2.0 Arrays (Affymetrix) according to standard protocols.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7396", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7396/samples/"}