{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 1587, "factors": [{"GSE7103GSM170876": {}}, {"GSE7103GSM170875": {}}, {"GSE7103GSM170870": {}}, {"GSE7103GSM170877": {}}, {"GSE7103GSM170868": {}}, {"GSE7103GSM170871": {}}, {"GSE7103GSM170873": {}}, {"GSE7103GSM170869": {}}, {"GSE7103GSM170874": {}}, {"GSE7103GSM170872": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "The aim of the study was to identify markers for the early diagnosis of endoprosthesis loosening, for the differentiation between wear-...", "pubmed_id": 17902171, "geo_gse_id": "E-GEOD-7103", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 10, "tags": ["cadherin", "disease", "e-cadherin", "serum", "thrombospondin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-samples-to-identi", "geo_id_plat": "E-GEOD-7103_A-AFFY-33", "name": "Transcription profiling of human samples to identify markers for early diagnosis of of endoprosthesis loosening", "created": "Jun.19, 2014", "summary": "The aim of the study was to identify markers for the early diagnosis of endoprosthesis loosening, for the differentiation between wear-particle induced and septic loosening, as well as to gather new insights into the pathogenesis. 824 genes were differentially expressed with a fold change greater than 2. Among these were Chitinase 1, CD52, Calpain 3, Apolipoprotein, CD18, Lysyl oxidase, Cathepsin D, E-Cadherin, VE-Cadherin, Nidogen, Angiopoietin 1 and Thrombospondin 2, and the differential expression levels were validated by RT-PCR. The chitinase activity was significantly higher in the blood from patients with wear-particle induced prosthesis loosening (p=0.001). However, using chitinase activity as a marker for early diagnosis, it has a specificity of 83% and a sensitivity of only 52%, due to a high variability both in the disease and in the control group. Experiment Overall Design: Gene expression profiles were generated from 5 periprosthetic membranes of wear-particle induced and 5 of infectious (septic) type using Affymetrix HG U133A oligonucleotide microarrays. The results of selected differentially expressed genes were validated by RT-PCR (n=30). The enzyme activity and the genotype of chitinase-1 were assessed in serum samples from 313 consecutive patients hospitalized for endoprosthesis loosening (n=54) or for other reasons, serving as control subjects (n=259).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7103", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-7103/samples/"}