{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE6909GSM159345": {}}, {"GSE6909GSM159346": {}}, {"GSE6909GSM159347": {}}, {"GSE6909GSM159348": {}}, {"GSE6909GSM159349": {}}, {"GSE6909GSM159350": {}}], "id": 7780, "pop_total": 0, "platform": 6, "summary_wrapped": "alpha2-adrenoceptors are essential presynaptic regulators of norepinephrine release from sympathetic nerves. Previous studies in mice...", "pubmed_id": 17673674, "geo_gse_id": "E-GEOD-6909", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["cell", "endothelial cell", "genome", "norepinephrine", "placenta"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-moise-placentae-e105-fr", "geo_id_plat": "E-GEOD-6909_A-AFFY-45", "name": "Transcription profiling of moise placentae (E10.5) from adra2bKO and adra2bWT mutants", "created": "Nov.13, 2014", "summary": "alpha2-adrenoceptors are essential presynaptic regulators of norepinephrine release from sympathetic nerves. Previous studies in mice with targeted deletions in the three alpha2-adrenoceptor genes have indicated that these receptors are essential for embryonic development. In the present study, we searched for the alpha2-adrenoceptor subtype(s) involved in placental development and its molecular mechanism using mice carrying targeted deletions in alpha2-adrenoceptor genes.  Congenic alpha2B-adrenoceptor-deficient mice (Adra2b-/-) developed a defect in fetal and maternal vessel formation in the placenta labyrinth at embryonic day E10.5. This defect was accompanied by reduced endothelial cell proliferation and decreased ERK1/2 phosphorylation levels in Adra2b-/- as compared with Adra2b+/+ placenta. Microarray analysis of wild-type and mutant placentae (maternal genotype Adra2b+/-) revealed 179 genes which were significantly up- or downregulated >1.5-fold in alpha2B-deficient placenta. The type 1 receptor for vascular endothelial growth factor (Flt1), which is coexpressed with alpha2B-adrenoceptors in spongiotrophoblast and giant cells of the placenta, was found to be 2.3-fold upregulated in alpha2B-deficient placenta. Neutralization of Flt1 and its soluble splice variant sFlt1 by a specific antibody in vivo prevented the vascular defect in alpha2B-deficient placenta at E10.5. Thus, alpha2B-adrenoceptors are essential to suppress antiangiogenic (s)Flt1 in spongiotrophoblasts to control the coordinated formation of a vascular labyrinth of fetal and maternal blood vessels in the murine placenta during development. Experiment Overall Design: Comparative transcriptome analysis was determined using the GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA, USA). Six microarrays from three wild-type and three adra2b deficient mice were performed.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6909", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6909/samples/"}