ArrayExpress Uploader0mouse7776arrayexpress_sid6To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to...E-GEOD-6875/profile/8773/arrayexpressuploader08cellproteinDec.12, 2014Falsetranscription-profiling-of-mouse-cd4-t-cells-expreE-GEOD-6875_A-AFFY-45Transcription profiling of mouse CD4+ T cells expressing Foxp3 FACS isolated from hemizygouse males to investigate development of regulatory t cell precursors in the absence of a functional foxp3 proteinNov.13, 2014To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to that of normal regulatory T cells Experiment Overall Design: Murine wild-type and mutant (delta) Foxp3 alleles were tagged with EGFP reporters. CD4 positive T cells expressing the respective tagged Foxp3 allele (Foxp3/EGFP and delta Foxp3/EGFP) were isolated from hemizygous male mice by cell sorting using FACS. CD4 positive cells that did not express the EGFP tag were a isolated. Total RNA was prepared and used in the array studies.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6875http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6875/samples/