ArrayExpress Uploader07771not specifiedMACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cellsB6 congenic for MHC Class II Ag7 (H2g7)not specifiedMACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cellsB6 congenic for MHC Class II Ag7 (H2g7)not specifiedMACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cellsB6 congenic for MHC Class II Ag7 (H2g7)not specifiedMACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cellsNODnot specifiedMACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cellsNODnot specifiedMACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cellsNOD94%MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cellsB6 congenic for MHC Class II Ag7 (H2g7)96%MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cellsB6 congenic for MHC Class II Ag7 (H2g7)96%MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cellsB6 congenic for MHC Class II Ag7 (H2g7)89%MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cellsNOD95%MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cellsNOD95%MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cellsNODarrayexpress_sid6The NOD (nonobese diabetic) mouse strain develops a characteristic autoimmune syndrome that closely resembles human type I diabetes. It...19073938E-GEOD-6813/profile/8773/arrayexpressuploader312cellsyndromeDec.12, 2014Falsegene-expression-profiles-of-cd4cd25-tregs-from-nodE-GEOD-6813_A-AFFY-45Gene expression profiles of CD4+CD25+ Tregs from NOD and B6.H2g7 miceNov.13, 2014The NOD (nonobese diabetic) mouse strain develops a characteristic autoimmune syndrome that closely resembles human type I diabetes. It has been suggested that NOD mice exhibit both numerical deficiency in CD4+CD25+ regulatory T cells (Treg) and reduced suppressive activity. We compared sorted CD4+CD25+ Tregs from the spleens of 6-7 week-old female NOD and nondiabetic B6.H2g7 mice. Tregs were 93±2% and 95±1% Foxp3+ in NOD and B6.H2g7 cells, respectively, on post-sort reanalysis. "Conventional" CD4+CD25- T cells (Tconv) are included as reference populations. Surprisingly, Treg "signature" is similar between the two strains, with only a few probesets that subtly deviate. Keywords: Cell population comparison from two mouse strains. For each strain (NOD and B6.g7), we analyzed two populations: CD4+CD25+ Treg and CD4+CD25- Tconv cells, for a total of four distinct populations. RNA from three mice were pooled for each replicate; there are three independent replicates for each population. After RMA normalization, intensity values were averaged across the three replicates and analyzed. We calculated the ratio of Treg/Tconv intensity values for each strain and compared the results.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6813mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6813/samples/