{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7771, "factors": [{"GSM155600": {"%FOXP3+ AFTER RESTAINING": "not specified", "SORTING": "MACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cells", "GENETIC BACKGROUND": "B6 congenic for MHC Class II Ag7 (H2g7)"}}, {"GSM155600": {"%FOXP3+ AFTER RESTAINING": "not specified", "SORTING": "MACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cells", "GENETIC BACKGROUND": "B6 congenic for MHC Class II Ag7 (H2g7)"}}, {"GSM155600": {"%FOXP3+ AFTER RESTAINING": "not specified", "SORTING": "MACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cells", "GENETIC BACKGROUND": "B6 congenic for MHC Class II Ag7 (H2g7)"}}, {"GSM155634": {"%FOXP3+ AFTER RESTAINING": "not specified", "SORTING": "MACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cells", "GENETIC BACKGROUND": "NOD"}}, {"GSM155634": {"%FOXP3+ AFTER RESTAINING": "not specified", "SORTING": "MACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cells", "GENETIC BACKGROUND": "NOD"}}, {"GSM155634": {"%FOXP3+ AFTER RESTAINING": "not specified", "SORTING": "MACS for CD25- cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25- cells", "GENETIC BACKGROUND": "NOD"}}, {"GSM155637": {"%FOXP3+ AFTER RESTAINING": "94%", "SORTING": "MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells", "GENETIC BACKGROUND": "B6 congenic for MHC Class II Ag7 (H2g7)"}}, {"GSM155638": {"%FOXP3+ AFTER RESTAINING": "96%", "SORTING": "MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells", "GENETIC BACKGROUND": "B6 congenic for MHC Class II Ag7 (H2g7)"}}, {"GSM155638": {"%FOXP3+ AFTER RESTAINING": "96%", "SORTING": "MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells", "GENETIC BACKGROUND": "B6 congenic for MHC Class II Ag7 (H2g7)"}}, {"GSM155640": {"%FOXP3+ AFTER RESTAINING": "89%", "SORTING": "MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells", "GENETIC BACKGROUND": "NOD"}}, {"GSM15564": {"%FOXP3+ AFTER RESTAINING": "95%", "SORTING": "MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells", "GENETIC BACKGROUND": "NOD"}}, {"GSM15564": {"%FOXP3+ AFTER RESTAINING": "95%", "SORTING": "MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells", "GENETIC BACKGROUND": "NOD"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The NOD (nonobese diabetic) mouse strain develops a characteristic autoimmune syndrome that closely resembles human type I diabetes.  It...", "pubmed_id": 19073938, "geo_gse_id": "E-GEOD-6813", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 12, "tags": ["cell", "syndrome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-profiles-of-cd4cd25-tregs-from-nod", "geo_id_plat": "E-GEOD-6813_A-AFFY-45", "name": "Gene expression profiles of CD4+CD25+ Tregs from NOD and B6.H2g7 mice", "created": "Nov.13, 2014", "summary": "The NOD (nonobese diabetic) mouse strain develops a characteristic autoimmune syndrome that closely resembles human type I diabetes.  It has been suggested that NOD mice exhibit both numerical deficiency in CD4+CD25+ regulatory T cells (Treg) and reduced suppressive activity.  We compared sorted CD4+CD25+ Tregs from the spleens of 6-7 week-old female NOD and nondiabetic B6.H2g7 mice.  Tregs were 93\u00b12% and 95\u00b11% Foxp3+ in NOD and B6.H2g7 cells, respectively, on post-sort reanalysis.  \"Conventional\" CD4+CD25- T cells (Tconv) are included as reference populations.  Surprisingly, Treg \"signature\" is similar between the two strains, with only a few probesets that subtly deviate. Keywords: Cell population comparison from two mouse strains. For each strain (NOD and B6.g7), we analyzed two populations: CD4+CD25+ Treg and CD4+CD25- Tconv cells, for a total of four distinct populations.  RNA from three mice were pooled for each replicate; there are three independent replicates for each population.  After RMA normalization, intensity values were averaged across the three replicates and analyzed.  We calculated the ratio of Treg/Tconv intensity values for each strain and compared the results.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6813", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6813/samples/"}