{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7769, "factors": [{"GSE6789GSM156802": {"Compound": "IL-1beta", "Genotype": "IRAK-4 deficient", "Time": 1}}, {"GSE6789GSM156802": {"Compound": "IL-1beta", "Genotype": "IRAK-4 deficient", "Time": 1}}, {"GSE6789GSM156804": {"Compound": "IL-1beta", "Genotype": "IRAK-4 deficient", "Time": 4}}, {"GSE6789GSM156804": {"Compound": "IL-1beta", "Genotype": "IRAK-4 deficient", "Time": 4}}, {"GSE6789GSM156800": {"Compound": "none", "Genotype": "IRAK-4 deficient", "Time": 4}}, {"GSE6789GSM156800": {"Compound": "none", "Genotype": "IRAK-4 deficient", "Time": 4}}, {"GSE6789GSM155304": {"Compound": "none", "Genotype": "wild_type", "Time": 4}}, {"GSE6789GSM156796": {"Compound": "IL-1beta", "Genotype": "wild_type", "Time": 1}}, {"GSE6789GSM156796": {"Compound": "IL-1beta", "Genotype": "wild_type", "Time": 1}}, {"GSE6789GSM156798": {"Compound": "IL-1beta", "Genotype": "wild_type", "Time": 4}}, {"GSE6789GSM156798": {"Compound": "IL-1beta", "Genotype": "wild_type", "Time": 4}}, {"GSE6789GSM155304": {"Compound": "none", "Genotype": "wild_type", "Time": 4}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "IRAK-4 is an essential component of the signal transduction complex downstream of the IL-1- and Toll-like receptors. Though regarded as...", "pubmed_id": 17337443, "geo_gse_id": "E-GEOD-6789", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 12, "tags": ["protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-embryonic-fibro-5", "geo_id_plat": "E-GEOD-6789_A-AFFY-45", "name": "Transcription profiling of mouse embryonic fibroblasts from wild type and IRAK-4 kinase dead animals stimulated with IL-1b for different durations to identify IRAK-4 kinase-dependent IL-1b response genes.", "created": "Nov.13, 2014", "summary": "IRAK-4 is an essential component of the signal transduction complex downstream of the IL-1- and Toll-like receptors. Though regarded as the first kinase in the signaling cascade, the role of IRAK-4 kinase activity versus its scaffold function is still controversial. In order to investigate the role of IRAK-4 kinase function in vivo, knock-in mice were generated by replacing the wild type IRAK-4 gene with a mutant gene encoding kinase deficient IRAK-4 protein (IRAK-4 KD). Analysis of embryonic fibroblasts and macrophages obtained from IRAK-4 KD mice with a number of experimental techniques demonstrated that they greatly lack responsiveness to stimulation with IL-1b or a Toll-like receptor 7 (TLR7) agonist. One of the techniques used, microarray analysis, identified IRAK-4 kinase-dependent IL-1b response genes in mouse embryonic fibroblasts and revealed that the induction of IL-1b-responsive mRNAs was largely ablated in IRAK-4 KD cells. In summary, our results suggest that IRAK-4 kinase activity plays a critical role in IL-1R/TLR7-mediated induction of inflammatory responses. Experiment Overall Design: The response of mouse embryonic fibroblasts from WT and IRAK4 kinase dead animals to stimulation with IL-1b at two time points was determined. There were 12 samples in total, 6 from WT and 6 from IRAK4 kinase dead cells; for each strain there were 3 conditions: growth for 4 hours without stimulation (the strain-specific control), growth for 1 hour with stimulation, and growth for 4 hours with stimulation; for each condition there were two biological replicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6789", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6789/samples/"}