ArrayExpress Uploader08797arrayexpress_sid8High cholesterol diet and xenobiotic treatment induce changes in cholesterol homeostasis and drug metabolism. Mice were either 7 days on...18261244E-GEOD-6721/profile/8773/arrayexpressuploader012liverDec.12, 2014Falsetranscription-profiling-of-mouse-liver-treated-w-2E-GEOD-6721_A-AFFY-36Transcription profiling of mouse liver treated with phenobarbital and fed a high cholesterol diet anNov.24, 2014High cholesterol diet and xenobiotic treatment induce changes in cholesterol homeostasis and drug metabolism. Mice were either 7 days on high cholesterol diet or were treated with phenobarbital. Liver samples were anayzed using Affymetrix GeneChip MOE430A. Experiment Overall Design: One group of mice was treated 50 mg/kg of phenobarbital in vehicle (5% DMSO in corn oil). Untreated group was injected vehicle only. Third group was 7 days on 1% (w/w) cholesterol diet prior vehicle treatment. After 10 hours animals were sacrificed and total RNA was isolated from liver. Total RNA from two animals was pooled resulting in 4 pools per group. These pools were analyzed using Affymetrix GeneChip MOE430A.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6721mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6721/samples/