{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7759, "factors": [{"GSE6674GSM154250": {}}, {"GSE6674GSM154251": {}}, {"GSE6674GSM154252": {}}, {"GSE6674GSM154244": {}}, {"GSE6674GSM154245": {}}, {"GSE6674GSM154246": {}}, {"GSE6674GSM154253": {}}, {"GSE6674GSM154254": {}}, {"GSE6674GSM154255": {}}, {"GSE6674GSM154247": {}}, {"GSE6674GSM154248": {}}, {"GSE6674GSM154249": {}}, {"GSE6674GSM154256": {}}, {"GSE6674GSM154257": {}}, {"GSE6674GSM154258": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "We have previously shown that rheumatoid factors (RF) produced by Fas-deficient autoimmune-prone mice typically bind autologous IgG2a...", "pubmed_id": 18025183, "geo_gse_id": "E-GEOD-6674", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 15, "tags": ["autoimmune disease", "chromatin", "compartment", "disease", "internal"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-am-14-b-cells-sti", "geo_id_plat": "E-GEOD-6674_A-AFFY-45", "name": "Transcription profiling of mouse AM-14 B cells stimulated through the B cell receptor (BCR) and/or Toll-like receptors (TLR)", "created": "Nov.13, 2014", "summary": "We have previously shown that rheumatoid factors (RF) produced by Fas-deficient autoimmune-prone mice typically bind autologous IgG2a with remarkably low affinity. Nevertheless, B cells representative of this RF population proliferate vigorously in response IgG2a/chromatin immune complexes through a mechanism dependent on the sequential engagement of the BCR and Toll-like receptor 9 (TLR9). To more precisely address the role of both receptors in this response, we analyzed the signaling pathways activated in AM14 B cells stimulated with these complexes. We found that the BCR not only serves to direct the chromatin complex to an internal compartment where it can engage TLR9 but also transmits a suboptimal signal that in combination with the signals emanating from TLR9 leads to NF?B activation and proliferation. Importantly, engagement of both receptors leads to the upregulation of a group of gene products, not induced by the BCR or TLR9 alone, that include IL-2. These data indicate that autoreactive B cells, stimulated by a combination of BCR and TLR9 ligands, acquire functional properties that may contribute to the activation of additional cells involved in the autoimmune disease process. Experiment Overall Design: 15 Samples, 3 replicates for each treatment", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6674", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-6674/samples/"}