ArrayExpress Uploader077294T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Metastatic4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Metastatic4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Metastatic4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Metastatic67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Non-metastatic67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Non-metastatic67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Non-metastatic67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Non-metastatic67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouseLung, Non-metastaticPBS controlLung, NormalPBS controlLung, NormalPBS controlLung, NormalPBS controlLung, NormalPBS controlLung, Normalarrayexpress_sid6Determine gene expression differences between normal, metastatic and non-metastatic mouse lung tissue. Priming of the organ-specific...21081700E-GEOD-62817/profile/8773/arrayexpressuploader214breastbreast carcinomacarcinomacellliverlunglung metastasisorganproteinvolumeDec.12, 2014Falsespr509-lung-metastasisE-GEOD-62817_A-AFFY-45SPR509: Lung metastasisNov.12, 2014Determine gene expression differences between normal, metastatic and non-metastatic mouse lung tissue. Priming of the organ-specific premetastatic sites is thought to be an important yet incompletely understood step during metastasis. In this study, we show that the metastatic tumors we examined overexpress granulocyte-colony stimulating factor (G-CSF), which expands and mobilizes Ly6G+Ly6C+ granulocytes and facilitates their subsequent homing at distant organs even before the arrival of tumor cells. Moreover, G-CSF-mobilized Ly6G+Ly6C+ cells produce the Bv8 protein, which has been implicated in angiogenesis and mobilization of myeloid cells. Anti-G-CSF or anti-Bv8 antibodies significantly reduced lung metastasis. Transplantation of Bv8 null fetal liver cells into lethally irradiated hosts also reduced metastasis. We identified an unexpected role for Bv8: the ability to stimulate tumor cell migration through activation of one of the Bv8 receptors, prokineticin receptor (PKR)-1. Finally, we show that administration of recombinant G-CSF is sufficient to increase the numbers of Ly6G+Ly6C+ cells in organ-specific metastatic sites and results in enhanced metastatic ability of several tumors. 67NR and 4T1mouse breast carcinoma cell lines were injected into fourth mammary fat pad; lung tissue was collected when tumor reached volume of 50mm3. RNA was extracted using Qiagen kit.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62817mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62817/samples/