{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7729, "factors": [{"GSM1533823": {"treatment": "4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Metastatic"}}, {"GSM1533823": {"treatment": "4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Metastatic"}}, {"GSM1533823": {"treatment": "4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Metastatic"}}, {"GSM1533823": {"treatment": "4T1 mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Metastatic"}}, {"GSM1533828": {"treatment": "67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Non-metastatic"}}, {"GSM1533828": {"treatment": "67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Non-metastatic"}}, {"GSM1533828": {"treatment": "67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Non-metastatic"}}, {"GSM1533828": {"treatment": "67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Non-metastatic"}}, {"GSM1533828": {"treatment": "67NR mouse breast carcinoma cell line injected into fourth mammary fat pad of BALB/c mouse", "organism part": "Lung, Non-metastatic"}}, {"GSM1533833": {"treatment": "PBS control", "organism part": "Lung, Normal"}}, {"GSM1533833": {"treatment": "PBS control", "organism part": "Lung, Normal"}}, {"GSM1533833": {"treatment": "PBS control", "organism part": "Lung, Normal"}}, {"GSM1533833": {"treatment": "PBS control", "organism part": "Lung, Normal"}}, {"GSM1533833": {"treatment": "PBS control", "organism part": "Lung, Normal"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Determine gene expression differences between normal, metastatic and non-metastatic mouse lung tissue. Priming of the organ-specific...", "pubmed_id": 21081700, "geo_gse_id": "E-GEOD-62817", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 14, "tags": ["breast", "breast carcinoma", "carcinoma", "cell", "liver", "lung", "lung metastasis", "organ", "protein", "volume"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "spr509-lung-metastasis", "geo_id_plat": "E-GEOD-62817_A-AFFY-45", "name": "SPR509: Lung metastasis", "created": "Nov.12, 2014", "summary": "Determine gene expression differences between normal, metastatic and non-metastatic mouse lung tissue. Priming of the organ-specific premetastatic sites is thought to be an important yet incompletely understood step during metastasis. In this study, we show that the metastatic tumors we examined overexpress granulocyte-colony stimulating factor (G-CSF), which expands and mobilizes Ly6G+Ly6C+ granulocytes and facilitates their subsequent homing at distant organs even before the arrival of tumor cells. Moreover, G-CSF-mobilized Ly6G+Ly6C+ cells produce the Bv8 protein, which has been implicated in angiogenesis and mobilization of myeloid cells. Anti-G-CSF or anti-Bv8 antibodies significantly reduced lung metastasis. Transplantation of Bv8 null fetal liver cells into lethally irradiated hosts also reduced metastasis. We identified an unexpected role for Bv8: the ability to stimulate tumor cell migration through activation of one of the Bv8 receptors, prokineticin receptor (PKR)-1. Finally, we show that administration of recombinant G-CSF is sufficient to increase the numbers of Ly6G+Ly6C+ cells in organ-specific metastatic sites and results in enhanced metastatic ability of several tumors. 67NR and 4T1mouse breast carcinoma cell lines were injected into fourth mammary fat pad; lung tissue was collected when tumor reached volume of 50mm3. RNA was extracted using Qiagen kit.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62817", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62817/samples/"}