ArrayExpress Uploaderarrayexpress_sidmouselymph nodesCD4+CD25+CD69- Tregslymph nodesCD4+CD25-CD69- Tcellslymph nodesCD4+CD25+CD69- Tregspancreatic lymph nodesCD4+CD25-CD69+ Tcellslymph nodesCD4+CD25+CD69- Tregspancreatic lymph nodesCD4+CD25+CD69+ Tregspancreatic lymph nodesCD4+CD25-CD69+ Tcellspancreatic lymph nodesCD4+CD25+CD69+ Tregslymph nodesCD4+CD25+CD69- Tregslymph nodesCD4+CD25-CD69- Tcellspancreatic lymph nodesCD4+CD25+CD69+ Tregslymph nodesCD4+CD25+CD69- Tregspancreatic lymph nodesCD4+CD25+CD69+ Tregspancreatic lymph nodesCD4+CD25-CD69+ Tcellslymph nodesCD4+CD25+CD69- Tregslymph nodesCD4+CD25-CD69- Tcells878208This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under...E-GEOD-62104/profile/8773/arrayexpressuploader216cellinsulinlymphDec.12, 2014FalseE-GEOD-62104_A-AFFY-36follicular-helper-t-cell-signature-in-type-1-diabeFollicular helper T cell signature in Type 1 DiabetesNov.24, 2014This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under the control of the rat insulin promoter and co-expression of the DO11.10 transgene (DO11 x rip-mOVA mice). The transcriptome of T cells isolated from the pancreatic lymph nodes (lymph nodes draining the site of self antigen expression) was compared with that of T cells isolated from inguinal lymph nodes (non-draining lymph nodes). T cells were sorted based on expression of CD4, DO11.10 TCR (KJ-126), CD25 and CD69. Primary cells from 6 week old DO11 x rip-mOVA mice were isolated ex-vivo from the pancreatic lymph nodes or inguinal lymph nodes. Cells were sorted by flow cytometry using antibodies to CD4, DO11.10 TCR (KJ-126+), CD25 and CD69. 3-6 replicates were collected per experimental group with each replicate deriving from 14 mice. RNA was isolated using the RNeasy micro kit (Qiagen).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62104http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62104/samples/