{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM1519673": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25+CD69- Tregs"}}, {"GSM1519674": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25-CD69- Tcells"}}, {"GSM1519673": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25+CD69- Tregs"}}, {"GSM1519676": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25-CD69+ Tcells"}}, {"GSM1519673": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25+CD69- Tregs"}}, {"GSM1519678": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25+CD69+ Tregs"}}, {"GSM1519676": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25-CD69+ Tcells"}}, {"GSM1519678": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25+CD69+ Tregs"}}, {"GSM1519673": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25+CD69- Tregs"}}, {"GSM1519674": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25-CD69- Tcells"}}, {"GSM1519678": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25+CD69+ Tregs"}}, {"GSM1519673": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25+CD69- Tregs"}}, {"GSM1519678": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25+CD69+ Tregs"}}, {"GSM1519676": {"TISSUE ORIGIN": "pancreatic lymph nodes", "CELL TYPE": "CD4+CD25-CD69+ Tcells"}}, {"GSM1519673": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25+CD69- Tregs"}}, {"GSM1519674": {"TISSUE ORIGIN": "lymph nodes", "CELL TYPE": "CD4+CD25-CD69- Tcells"}}], "id": 8782, "pop_total": 0, "platform": 8, "summary_wrapped": "This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under...", "geo_gse_id": "E-GEOD-62104", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 16, "tags": ["cell", "insulin", "lymph"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-62104_A-AFFY-36", "slug": "follicular-helper-t-cell-signature-in-type-1-diabe", "geo_gds_id": "", "name": "Follicular helper T cell signature in Type 1 Diabetes", "created": "Nov.24, 2014", "summary": "This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under the control of the rat insulin promoter and co-expression of the DO11.10 transgene (DO11 x rip-mOVA mice). The transcriptome of T cells isolated from the pancreatic lymph nodes (lymph nodes draining the site of self antigen expression) was compared with that of T cells isolated from inguinal lymph nodes (non-draining lymph nodes). T cells were sorted based on expression of CD4, DO11.10 TCR (KJ-126), CD25 and CD69. Primary cells from 6 week old DO11 x rip-mOVA mice were isolated ex-vivo from the pancreatic lymph nodes or inguinal lymph nodes. Cells were sorted by flow cytometry using antibodies to CD4, DO11.10 TCR (KJ-126+), CD25 and CD69.  3-6 replicates were collected per experimental group with each replicate deriving from 14 mice. RNA was isolated using the RNeasy micro kit (Qiagen).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62104", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-62104/samples/"}