{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM1496307 1": {}}, {"GSM1496308 1": {}}, {"GSM1496309 1": {}}, {"GSM1496310 1": {}}, {"GSM1496311 1": {}}, {"GSM1496312 1": {}}, {"GSM1496313 1": {}}, {"GSM1496314 1": {}}, {"GSM1496315 1": {}}, {"GSM1496316 1": {}}, {"GSM1496317 1": {}}, {"GSM1496318 1": {}}, {"GSM1496319 1": {}}, {"GSM1496320 1": {}}, {"GSM1496321 1": {}}, {"GSM1496322 1": {}}, {"GSM1496323 1": {}}], "id": 7707, "pop_total": 0, "platform": 6, "summary_wrapped": "Mouse adult female brain\u2019s cortex (C57BL/6, Charles River Laboratories, Inc.) was isolated and stored immediately at -80\u00b0C. Subsequently,...", "geo_gse_id": "E-GEOD-61081", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 17, "tags": ["cell", "cortex"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-61081_A-AFFY-45", "slug": "dilution-and-amplification-control-study", "geo_gds_id": "", "name": "Dilution and amplification control study", "created": "Nov.12, 2014", "summary": "Mouse adult female brain\u2019s cortex (C57BL/6, Charles River Laboratories, Inc.) was isolated and stored immediately at -80\u00b0C. Subsequently, the mRNA (15\u00b5g) was isolated using TRIzol Reagent and MicroFastTrack 2.0 Kit (Invitrogen). A Sample of 5\u00b5g was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently aRNA amplified for a total of 2 and 4 rounds and assessed on Affymetrix Mouse 430.2 arrays. A Sample of 5\u00b5g of mRNA was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently amplified, as described above, for a total of 2 and 4 rounds and assessed on Affymetrix Mouse 430.2 arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-61081", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-61081/samples/"}