{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1483696": {"CELL TYPE": "Regulatory T cells (CD4+CD25hiFoxp3rfp+, Treg)"}}, {"GSM1483696": {"CELL TYPE": "Regulatory T cells (CD4+CD25hiFoxp3rfp+, Treg)"}}, {"GSM1483696": {"CELL TYPE": "Regulatory T cells (CD4+CD25hiFoxp3rfp+, Treg)"}}, {"GSM1483699": {"CELL TYPE": "Exosome"}}, {"GSM1483699": {"CELL TYPE": "Exosome"}}, {"GSM1483699": {"CELL TYPE": "Exosome"}}, {"GSM1483702": {"CELL TYPE": "Dicer KO cells"}}, {"GSM1483702": {"CELL TYPE": "Dicer KO cells"}}, {"GSM1483702": {"CELL TYPE": "Dicer KO cells"}}, {"GSM1483705": {"CELL TYPE": "co-cultured Dicer KO cells with Treg cells"}}, {"GSM1483705": {"CELL TYPE": "co-cultured Dicer KO cells with Treg cells"}}, {"GSM1483705": {"CELL TYPE": "co-cultured Dicer KO cells with Treg cells"}}], "id": 7694, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Foxp3+ regulatory T (Treg) cells prevent inflammatory disease but the mechanistic basis of suppression is not understood completely ....", "geo_gse_id": "E-GEOD-60615", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["cell", "cytokine", "disease"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "mirnas-in-treg-derived-exosomes", "geo_id_plat": "E-GEOD-60615_A-AFFY-45", "name": "miRNAs in Treg-derived Exosomes", "created": "Nov.12, 2014", "summary": "Foxp3+ regulatory T (Treg) cells prevent inflammatory disease but the mechanistic basis of suppression is not understood completely . Gene silencing by RNA interference can act in a cell-autonomous and non-cell-autonomous manner, providing mechanisms of inter-cellular regulation. Here, we demonstrate that non-cell-autonomous gene silencing, mediated by miRNA-containing exosomes, is a mechanism employed by Treg cells to suppress T cell-mediated disease. Treg cells transferred microRNAs (miRNA) to various immune cells, including T helper 1 (Th1) cells, suppressing Th1 cell proliferation and cytokine secretion. Use of Dicer-deficient or Rab27a and Rab27b double-deficient Treg cells to disrupt miRNA-biogenesis or the exosomal pathway, respectively, established a requirement for miRNAs and exosomes for Treg cell-mediated suppression. Transcriptional analysis and miRNA inhibitor studies showed that exosome-mediated transfer of Let-7d from Treg cell to Th1 cells contributed to suppression and prevention of systemic disease. These studies reveal a mechanism of Treg cell-mediated suppression mediated by miRNA-containing exosomes. Regulatory T cells (CD4+CD25hiFoxp3rfp+, Treg) were isolated from naive mice. RNA as extracted form some Treg cells, while others were cultured in complete IMDM media for 3 days, stimulated with anti-CD3 anti-CD3 (1ug/ml) and anti-CD28 (10ug/ml). Exosomes were recovered from Treg cell supernatant, as described, and RNA was extracted form the purified exosomes. To identify which miRNAs were transferred to Dicer-deficient (KO) cells from Treg cells, we cultured Dicer KO cells alone, or co-cultured Dicer KO cells with Treg cells. RNA was extracted form Dicer KO cells cultured alone or from Dicer KO cells cultured in the presence of Treg cells. 3 x biological replicates were used. Each biological replicate was derived from a pool of 3-5 samples.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-60615", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-60615/samples/"}