{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1468777": {"CALCINEURIN GENE CNB1 DELETED": "no", "TREATMENT": "corn oil"}}, {"GSM1468777": {"CALCINEURIN GENE CNB1 DELETED": "no", "TREATMENT": "corn oil"}}, {"GSM1468777": {"CALCINEURIN GENE CNB1 DELETED": "no", "TREATMENT": "corn oil"}}, {"GSM1468777": {"CALCINEURIN GENE CNB1 DELETED": "no", "TREATMENT": "corn oil"}}, {"GSM1468777": {"CALCINEURIN GENE CNB1 DELETED": "no", "TREATMENT": "corn oil"}}, {"GSM1468782": {"CALCINEURIN GENE CNB1 DELETED": "yes", "TREATMENT": "tamoxifen"}}, {"GSM1468782": {"CALCINEURIN GENE CNB1 DELETED": "yes", "TREATMENT": "tamoxifen"}}, {"GSM1468782": {"CALCINEURIN GENE CNB1 DELETED": "yes", "TREATMENT": "tamoxifen"}}, {"GSM1468782": {"CALCINEURIN GENE CNB1 DELETED": "yes", "TREATMENT": "tamoxifen"}}, {"GSM1468782": {"CALCINEURIN GENE CNB1 DELETED": "yes", "TREATMENT": "tamoxifen"}}], "id": 7688, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Activated NOTCH1 induces T-ALL in mice when transduced in bone marrow (BM) cells. T-ALL cells activate the calcineurin/NFAT pathway in...", "geo_gse_id": "E-GEOD-60258", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 10, "tags": ["bone", "bone marrow", "leukemia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "calcineurin-dependent-transcriptome-in-icn1-activa", "geo_id_plat": "E-GEOD-60258_A-AFFY-45", "name": "Calcineurin-dependent transcriptome in ICN1 (activated NOTCH1)-induced T cell acute lymphoblastic leukemia (T-ALL)", "created": "Nov.12, 2014", "summary": "Activated NOTCH1 induces T-ALL in mice when transduced in bone marrow (BM) cells. T-ALL cells activate the calcineurin/NFAT pathway in vivo (Medyouf H. et al. Nat Med 2007 [PMID 17515895]). Through appropriate crosses, mouse ICN1-induced T-ALL were generated that include a ROSCreERT2 transgene and 2 copies of a CnB1 floxed alleles. Cohorts of secondary recipient mice were injected with these T-ALL cells, and when becoming leukemic, divided into 2 subgroups. Group 1 was treated with Tamoxifen (TAM), resulting in CnB1 gene deletion and inactivation of calcineurin. Group 2 was treated with carrier solvent (corn OIL), thus maintaining CnB1 and calcineurin activity. RNAs were extracted from flow cytometry-sorted leukemic cells. Details in Gachet S. et al. Leukemia 2013 (PMID 23689515).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-60258", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-60258/samples/"}