{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE5959GSM138292": {"Phenotype": "low serum IGF-I", "StrainOrLine": "B6.C3H-6T"}}, {"GSE5959GSM138292": {"Phenotype": "low serum IGF-I", "StrainOrLine": "B6.C3H-6T"}}, {"GSE5959GSM138292": {"Phenotype": "low serum IGF-I", "StrainOrLine": "B6.C3H-6T"}}, {"GSE5959GSM138289": {"Phenotype": "wild type", "StrainOrLine": "C57BL/6J"}}, {"GSE5959GSM138289": {"Phenotype": "wild type", "StrainOrLine": "C57BL/6J"}}, {"GSE5959GSM138289": {"Phenotype": "wild type", "StrainOrLine": "C57BL/6J"}}], "id": 7677, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5959", "summary_wrapped": "Several studies have shown that bone mineral density (BMD), a clinically measurable predictor of osteoporotic fracture, is the sum of...", "pubmed_id": 17584978, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 6, "tags": ["bone", "chromosome", "genome", "liver", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-livers-from-female-c57b", "geo_id_plat": "E-GEOD-5959_A-AFFY-45", "name": "Transcription profiling of livers from female C57BL/6J and B6.C3H-6T mice to study differential gene expression in the two strains.", "created": "Nov.12, 2014", "summary": "Several studies have shown that bone mineral density (BMD), a clinically measurable predictor of osteoporotic fracture, is the sum of genetic and environmental influences.  In addition, serum IGF-1 levels have been correlated to both BMD and fracture risk.  We previously identified a Quantitative Trait Locus (QTL) for Bone Mineral Density (BMD) on mouse Chromosome (Chr) 6 that overlaps a QTL for serum IGF-1.  The B6.C3H-6T (6T) congenic mouse is homozygous for C57BL/6J (B6) alleles across the genome except for a 30 cM region on Chr 6 that is homozygous for C3H/HeJ (C3H) alleles.  This mouse was created to study biology behind both the BMD and the serum IGF-1 QTLs and to identify the gene(s) underlying these QTLs.   Female 6T mice have lower BMD and lower serum IGF-1 levels at all ages measured. As the liver is the major source of serum IGF-1, we examined differential expression in the livers of fasted female B6 and 6T mice by microarray. Experiment Overall Design: The experimental design of this experiment was a simple two-factor experiment, with three biological replicates of each factor (in this case mouse strain, B6.C3H-6T vs. C57BL/6J).  Each sample represented one mouse; no samples were pooled.   No technical replicated were done.", "geo_gse_id": "E-GEOD-5959", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-5959/samples/"}