{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1431110 1": {}}, {"GSM1431111 1": {}}, {"GSM1431112 1": {}}, {"GSM1431113 1": {}}, {"GSM1431114 1": {}}, {"GSM1431115 1": {}}, {"GSM1431116 1": {}}, {"GSM1431117 1": {}}, {"GSM1431118 1": {}}, {"GSM1431119 1": {}}, {"GSM1431120 1": {}}, {"GSM1431121 1": {}}, {"GSM1431122 1": {}}, {"GSM1431123 1": {}}, {"GSM1431124 1": {}}, {"GSM1431125 1": {}}, {"GSM1431126 1": {}}, {"GSM1431127 1": {}}], "id": 4779, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The ontogeny of human Langerhans cells (LCs) remains poorly characterized, in particular the nature of LC precursors and the factors that...", "geo_gse_id": "E-GEOD-59237", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 18, "tags": ["dendritic", "skin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "tslp-effects-on-primary-human-blood-dendritic-cell", "geo_id_plat": "E-GEOD-59237_A-AFFY-44", "name": "TSLP effects on primary human blood dendritic cells", "created": "Sep.21, 2014", "summary": "The ontogeny of human Langerhans cells (LCs) remains poorly characterized, in particular the nature of LC precursors and the factors that may drive LC differentiation. Through a systematic transcriptomic analysis of TSLP-activated dendritic cells (DCs), we unexpectedly identified markers that have been associated with a skin-homing potential as well as with a LC phenotype. We performed transcriptomic analysis of TSLP-activated blood DCs, as compared to freshly purified, Medium-, and TNF-activated DCs. Among TSLP up-regulated genes, we identified molecules associated with skin homing, LC phenotype, and LC function, as determined by a literature-based survey. Conversely, genes not expressed in LCs were not found among TSLP-induced genes. Further experiments showed that TGF-\u03b2 synergized with TSLP leading to the differentiation of blood BDCA-1+ DCs into bona fide Birbeck granule-positive LCs. The ontogeny of human Langerhans cells (LCs) remains poorly characterized, in particular the nature of LC precursors and the factors that may drive LC differentiation. Through a systematic transcriptomic analysis of TSLP-activated dendritic cells (DCs), we unexpectedly identified markers that have been associated with a skin-homing potential as well as with a LC phenotype. We performed transcriptomic analysis of TSLP-activated blood DCs, as compared to freshly purified, Medium-, and TNF-activated DCs. Among TSLP up-regulated genes, we identified molecules associated with skin homing, LC phenotype, and LC function, as determined by a literature-based survey. Conversely, genes not expressed in LCs were not found among TSLP-induced genes. Further experiments showed that TGF-\u03b2 synergized with TSLP leading to the differentiation of blood BDCA-1+ DCs into bona fide Birbeck granule-positive LCs. Dendritic cells (DC) were purified from the blood of healthy human donors. Each replicate comes from a different donor. There are 6 freshly purified samples (Control_0h),  4  DC samples cultured during 6h with no additional stimulus than the medium (Control_6h), 5 samples cultured during 6h with recombinant TSLP (Thymic Stromal Lymphopoietin at 20 ng/uL) (TSLP_6h) and 3 samples cultured during 6h with recombinant TNF (Tumor necrosis factor at 20 ng/mL) (TNF_6h).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-59237", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-59237/samples/"}