{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1430397": {"DEVELOPMENTAL STAGE": "peripherally induced Treg", "CELL POPULATION": "GFP-RFP+"}}, {"GSM1430397": {"DEVELOPMENTAL STAGE": "peripherally induced Treg", "CELL POPULATION": "GFP-RFP+"}}, {"GSM1430399": {"DEVELOPMENTAL STAGE": "thymically induced Treg", "CELL POPULATION": "GFP+RFP+"}}, {"GSM1430399": {"DEVELOPMENTAL STAGE": "thymically induced Treg", "CELL POPULATION": "GFP+RFP+"}}, {"GSM1430397": {"DEVELOPMENTAL STAGE": "peripherally induced Treg", "CELL POPULATION": "GFP-RFP+"}}, {"GSM1430397": {"DEVELOPMENTAL STAGE": "peripherally induced Treg", "CELL POPULATION": "GFP-RFP+"}}, {"GSM1430399": {"DEVELOPMENTAL STAGE": "thymically induced Treg", "CELL POPULATION": "GFP+RFP+"}}, {"GSM1430399": {"DEVELOPMENTAL STAGE": "thymically induced Treg", "CELL POPULATION": "GFP+RFP+"}}], "id": 7671, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Here, we report on experiments in double-transgenic mice, in which RFP is expressed in all Foxp3+ Treg cells, whereas Foxp3-dependent GFP...", "geo_gse_id": "E-GEOD-59202", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["lymph", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "fluorochrome-based-definition-of-naturally-occurri", "geo_id_plat": "E-GEOD-59202_A-AFFY-45", "name": "Fluorochrome-based definition of naturally occurring Foxp3+ regulatory T cells of intra- and extrathymic origin", "created": "Nov.12, 2014", "summary": "Here, we report on experiments in double-transgenic mice, in which RFP is expressed in all Foxp3+ Treg cells, whereas Foxp3-dependent GFP expression is exclusively confined to intrathymically induced Foxp3+ Treg cells. This novel molecular genetic tool enabled us to faithfully track and characterize naturally induced Treg cells of intrathymic (RFP+GFP+) and extrathymic (RFP+GFP\u2212) origin in otherwise unmanipulated mice. These experiments directly demonstrate that extrathymically induced Treg cells substantially contribute to the overall pool of mature Foxp3+ Treg cells residing in peripheral lymphoid tissues of steady-state mice. Furthermore, we provide evidence that intra- and extrathymically induced Foxp3+ Treg cells represent distinct phenotypic and functional sublineages. CD4+CD25+ RFP+GFP- and CD4+CD25+ RFP+GFP+ T cells from pooled lymph nodes and pooled spleens of 20 mice were FACS sorted for RNA extraction and hybridization on Affymetrix microarrays in duplicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-59202", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-59202/samples/"}